Bacterial extracellular vesicles in biofluids as potential diagnostic biomarkers
Introduction: Extracellular vesicles (EVs) are spherical membrane-derived lipid bilayers released by cells. The human microbiota consists of trillions of microorganisms, with bacteria being the largest group secreting microbial EVs. The discovery of bacterial EVs (BEVs) has garnered interest among r...
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2022
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my.sunway.eprints.30032024-08-06T01:49:46Z http://eprints.sunway.edu.my/3003/ Bacterial extracellular vesicles in biofluids as potential diagnostic biomarkers Su, Kar Yan Koh Kok, Jie Yi Chua, Yie Wei Ong, Shearn Dior Ser, Hooi Leng * Priyia, Pusparajah Saw, Pui San Goh, Bey Hing * Lee, Wai Leng QH Natural history QR Microbiology R Medicine (General) Introduction: Extracellular vesicles (EVs) are spherical membrane-derived lipid bilayers released by cells. The human microbiota consists of trillions of microorganisms, with bacteria being the largest group secreting microbial EVs. The discovery of bacterial EVs (BEVs) has garnered interest among researchers as potential diagnostic markers, given that the microbiota is known to be associated with various diseases and EVs carry important macromolecular cargo for intercellular interaction. Areas covered: The differential bacterial composition identified from BEVs isolated from biofluids between patients and healthy controls may be valuable for detecting diseases. Therefore, BEVs may serve as novel diagnostic markers. Literature search on PubMed and Google Scholar databases was conducted. In this special report, we outline the commonly used approach for investigating BEVs in biofluids, the 16S ribosomal RNA gene sequencing of V3-V4 hypervariable regions, and the recent studies exploring the potential of BEVs as biomarkers for various diseases. Expert opinion: The emerging field of BEVs offers new possibilities for the diagnosis of various types of diseases, although there remain issues that need to be resolved in this research area to implement BEVs in clinical applications. Hence, it is important for future studies to take these challenges into consideration when investigating the diagnostic value of BEVs. Taylor and Francis Group 2022 Article PeerReviewed Su, Kar Yan and Koh Kok, Jie Yi and Chua, Yie Wei and Ong, Shearn Dior and Ser, Hooi Leng * and Priyia, Pusparajah and Saw, Pui San and Goh, Bey Hing * and Lee, Wai Leng (2022) Bacterial extracellular vesicles in biofluids as potential diagnostic biomarkers. Expert Review of Molecular Diagnostics, 22 (12). pp. 1057-1062. ISSN 1744-8352 https://doi.org/10.1080/14737159.2022.2166403 10.1080/14737159.2022.2166403 |
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QH Natural history QR Microbiology R Medicine (General) Su, Kar Yan Koh Kok, Jie Yi Chua, Yie Wei Ong, Shearn Dior Ser, Hooi Leng * Priyia, Pusparajah Saw, Pui San Goh, Bey Hing * Lee, Wai Leng Bacterial extracellular vesicles in biofluids as potential diagnostic biomarkers |
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Introduction: Extracellular vesicles (EVs) are spherical membrane-derived lipid bilayers released by cells. The human microbiota consists of trillions of microorganisms, with bacteria being the largest group secreting microbial EVs. The discovery of bacterial EVs (BEVs) has garnered interest among researchers as potential diagnostic markers, given that the microbiota is known to be associated with various diseases and EVs carry important macromolecular cargo for intercellular interaction.
Areas covered: The differential bacterial composition identified from BEVs isolated from biofluids between patients and healthy controls may be valuable for detecting diseases. Therefore, BEVs may serve as novel diagnostic markers. Literature search on PubMed and Google Scholar databases was conducted. In this special report, we outline the commonly used approach for investigating BEVs in biofluids, the 16S ribosomal RNA gene sequencing of V3-V4 hypervariable regions, and the recent studies exploring the potential of BEVs as biomarkers for various diseases.
Expert opinion: The emerging field of BEVs offers new possibilities for the diagnosis of various types of diseases, although there remain issues that need to be resolved in this research area to implement BEVs in clinical applications. Hence, it is important for future studies to take these challenges into consideration when investigating the diagnostic value of BEVs. |
format |
Article |
author |
Su, Kar Yan Koh Kok, Jie Yi Chua, Yie Wei Ong, Shearn Dior Ser, Hooi Leng * Priyia, Pusparajah Saw, Pui San Goh, Bey Hing * Lee, Wai Leng |
author_facet |
Su, Kar Yan Koh Kok, Jie Yi Chua, Yie Wei Ong, Shearn Dior Ser, Hooi Leng * Priyia, Pusparajah Saw, Pui San Goh, Bey Hing * Lee, Wai Leng |
author_sort |
Su, Kar Yan |
title |
Bacterial extracellular vesicles in biofluids as potential diagnostic biomarkers |
title_short |
Bacterial extracellular vesicles in biofluids as potential diagnostic biomarkers |
title_full |
Bacterial extracellular vesicles in biofluids as potential diagnostic biomarkers |
title_fullStr |
Bacterial extracellular vesicles in biofluids as potential diagnostic biomarkers |
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Bacterial extracellular vesicles in biofluids as potential diagnostic biomarkers |
title_sort |
bacterial extracellular vesicles in biofluids as potential diagnostic biomarkers |
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Taylor and Francis Group |
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2022 |
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http://eprints.sunway.edu.my/3003/ https://doi.org/10.1080/14737159.2022.2166403 |
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