Agrobacterium rhizogenes - mediated hairy roots cultures of capsicum annuum and capsicum frutescens / Nursuria Md Setamam

• Main Author: Md Setamam, Nursuria
• Format: Thesis
• Language: English
• Published: 2015
• Subjects: SB Plant culture
• Online Access: https://ir.uitm.edu.my/id/eprint/18446/2/TM_NURSURIA%20MD%20SETAMAM%20AS%2015_5.pdf; https://ir.uitm.edu.my/id/eprint/18446/
• Institution: Universiti Teknologi Mara

Economic values as crops and medicinal plants. In recent years, the establishment of hairy roots cultures via Agrobacterium rhizogenes had provided numerous advantages to many plant studies including Capsicum species. Therefore, in this research, A. rhizogenes— mediated hairy roots cultures were studied with the expectation to provide a better understanding and alternative solutions regarding these species. The objectives were to induce and proliferate the putative hairy roots, callus, and regenerated plantlets of C. annuum and C. frutescens with variables factors such as hormones, media and explants. The volatile compounds present in cultures samples were also identified. Initially, the cotyledon, radical and hypocotyl explants were obtained from seedlings and co-cultured with isolated strains of A. rhizogenes (ATCC 15834, ATCC 43056, ATCC 13333 and ATCC 43057). The highest induction putative hairy roots were transferred as explants into liquid MS with 0.2, 0.4, 0.6, 0.8, 1.0 mg/L of IAA for proliferation. The explants were also cultured in 0.5, 1.0, 1.5, 2.0 mg/L of each IAA, NAA, and 2,4-D for callus induction. In plant regeneration, the explants were cultured in solid MS containing 0.25, 0.5, 1.0, 1.5, 2.0 mg/L of BAP. Each treatment consisted of 20 replications and subcultured every two weeks. Proliferated hairy roots, callus, leaves, and stem from putative hairy roots explants were dried and extracted by using three types of solvents for GC-MS analysis. Findings showed that, the highest induction efficiency was achieved by using cotyledon explants which were ATCC 43056 for C. annuum and ATCC 15834 for C. frutescens. The presence of 1.0 mg/L IAA was suitable for putative hairy roots proliferation in both species. Meanwhile, 1.0 mg/ L and 1.5 mg/L of NAA for C. annuum and C. frutescens respectively, improved the callus induction. Through direct morphogenesis, the optimum concentration of BAP for plant regeneration were achieved in 1.5 mg/L for C. annuum and 1.0 mg/L for C. frutescens. Various beneficial compounds had been identified via GC-MS analysis for both C. annuum (33 compounds) and C. frutescens (37 compounds). More compounds were found in hexane extract compared to other solvents and proliferated putative hairy roots consisted more of compounds than other cultures. As a conclusion, A. rhizogenes—mediated hairy roots cultures can be used as an alternative tool to overcome recalcitrant. However, more further studies on additional factors were recommended in the future.