Review on genetic transformation of rice / Nur Amalina Ghazali

Rice or its scientific name, Oryza sativa L. is one of the world’s valuable crops. It is cultivated worldwide on 8.2 million hectares in 58 countries annually. Rice is a dietary staple food and one of the most important cereal crops, especially for people in Asia. A switch in production and consumpt...

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Main Author: Ghazali, Nur Amalina
Format: Student Project
Language:English
Published: Faculty of Plantation and Agrotechnology 2019
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Online Access:http://ir.uitm.edu.my/id/eprint/24405/1/24405.pdf
http://ir.uitm.edu.my/id/eprint/24405/
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Institution: Universiti Teknologi Mara
Language: English
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spelling my.uitm.ir.244052020-07-20T07:08:50Z http://ir.uitm.edu.my/id/eprint/24405/ Review on genetic transformation of rice / Nur Amalina Ghazali Ghazali, Nur Amalina Genetics Rice or its scientific name, Oryza sativa L. is one of the world’s valuable crops. It is cultivated worldwide on 8.2 million hectares in 58 countries annually. Rice is a dietary staple food and one of the most important cereal crops, especially for people in Asia. A switch in production and consumption patterns would improve food security where food grains are scarce, and provide more health beneficial food components, may prevent some diseases and ease the burden on the earth. Modern applications of agricultural biotechnology involves the development of gene transfer methods for the genetic transformation to increase number of important crops in daily life especially rice. The establishment of an efficient genetic transformation protocol for rice is important in improvement of traits. Despite a variety of available DNA delivery methods, Agrobacterium and biolistic mediated transformation remain two predominantly applied approaches. Most of the studies suggested that the transformation efficiency of the indica rice varieties is unsatisfactory in comparison to japonica cultivars as majority of the indica rice varieties are recalcitrant to in vitro responses. For efficient plant transformation, the selection of actively growing regenerable calli and the optimization of the culture conditions for the co-cultivation of rice calli with Agrobacterium are the most important factors. Acetosyringone was found as an essential key factor in achieving the rice transformation. Maximum rice transformation efficiency was achieved with the presence of acetosyringone at 100 μM. In conclusion, the factors that affecting the transformation efficiency of rice have been determined in this review. Faculty of Plantation and Agrotechnology 2019 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/24405/1/24405.pdf Ghazali, Nur Amalina (2019) Review on genetic transformation of rice / Nur Amalina Ghazali. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Genetics
spellingShingle Genetics
Ghazali, Nur Amalina
Review on genetic transformation of rice / Nur Amalina Ghazali
description Rice or its scientific name, Oryza sativa L. is one of the world’s valuable crops. It is cultivated worldwide on 8.2 million hectares in 58 countries annually. Rice is a dietary staple food and one of the most important cereal crops, especially for people in Asia. A switch in production and consumption patterns would improve food security where food grains are scarce, and provide more health beneficial food components, may prevent some diseases and ease the burden on the earth. Modern applications of agricultural biotechnology involves the development of gene transfer methods for the genetic transformation to increase number of important crops in daily life especially rice. The establishment of an efficient genetic transformation protocol for rice is important in improvement of traits. Despite a variety of available DNA delivery methods, Agrobacterium and biolistic mediated transformation remain two predominantly applied approaches. Most of the studies suggested that the transformation efficiency of the indica rice varieties is unsatisfactory in comparison to japonica cultivars as majority of the indica rice varieties are recalcitrant to in vitro responses. For efficient plant transformation, the selection of actively growing regenerable calli and the optimization of the culture conditions for the co-cultivation of rice calli with Agrobacterium are the most important factors. Acetosyringone was found as an essential key factor in achieving the rice transformation. Maximum rice transformation efficiency was achieved with the presence of acetosyringone at 100 μM. In conclusion, the factors that affecting the transformation efficiency of rice have been determined in this review.
format Student Project
author Ghazali, Nur Amalina
author_facet Ghazali, Nur Amalina
author_sort Ghazali, Nur Amalina
title Review on genetic transformation of rice / Nur Amalina Ghazali
title_short Review on genetic transformation of rice / Nur Amalina Ghazali
title_full Review on genetic transformation of rice / Nur Amalina Ghazali
title_fullStr Review on genetic transformation of rice / Nur Amalina Ghazali
title_full_unstemmed Review on genetic transformation of rice / Nur Amalina Ghazali
title_sort review on genetic transformation of rice / nur amalina ghazali
publisher Faculty of Plantation and Agrotechnology
publishDate 2019
url http://ir.uitm.edu.my/id/eprint/24405/1/24405.pdf
http://ir.uitm.edu.my/id/eprint/24405/
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