Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar
Polymerase Chain Reaction (PCR) analysis in the study of oil palm DNA generally carried out by using DNA template obtained from grinding of leaf samples in liquid nitrogen followed by hexadecyltrimethylammonium bromide (CTAB) protocol. The present study explores the FTA card as a method to retrieve...
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my.uitm.ir.282612020-02-11T10:00:26Z http://ir.uitm.edu.my/id/eprint/28261/ Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar Wan Hanafi, Wan Nurhayati Abu Bakar, Nur Syahirah Animal biochemistry DNA. Deoxyribonucleic acids Polymerase Chain Reaction (PCR) analysis in the study of oil palm DNA generally carried out by using DNA template obtained from grinding of leaf samples in liquid nitrogen followed by hexadecyltrimethylammonium bromide (CTAB) protocol. The present study explores the FTA card as a method to retrieve PCR-amplifiable oil palm DNA. Oil palm leaves were cut and crushed before deposited onto the FTA card. An attempt was made by amplifying the EgSHP gene using a punch of FTA card as a DNA template. The successful outcome of PCR was measured by the presence of PCR amplicons on 1% agarose gel electrophoresis, indicate the genotype of oil palm fruit form. This present study demonstrates that the FTA card provides a versatile alternative to the study of oil palm genetics. Penerbit UiTM (UiTM Press) 2019 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/28261/1/28261.pdf Wan Hanafi, Wan Nurhayati and Abu Bakar, Nur Syahirah (2019) Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar. Scientific Research Journal, 16 (2). pp. 17-26. ISSN 1675-7009 https://srj.uitm.edu.my/ https://doi.org/10.24191/srj.v16i2.6306 |
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Animal biochemistry DNA. Deoxyribonucleic acids Wan Hanafi, Wan Nurhayati Abu Bakar, Nur Syahirah Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar |
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Polymerase Chain Reaction (PCR) analysis in the study of oil palm DNA generally carried out by using DNA template obtained from grinding of leaf samples in liquid nitrogen followed by hexadecyltrimethylammonium bromide (CTAB) protocol. The present study explores the FTA card as a method to retrieve PCR-amplifiable oil palm DNA. Oil palm leaves were cut and crushed before deposited onto the FTA card. An attempt was made by amplifying the EgSHP gene using a punch of FTA card as a DNA template. The successful outcome of PCR was measured by the presence of PCR amplicons on 1% agarose gel electrophoresis, indicate the genotype of oil palm fruit form. This present study demonstrates that the FTA card provides a versatile alternative to the study of oil palm genetics. |
format |
Article |
author |
Wan Hanafi, Wan Nurhayati Abu Bakar, Nur Syahirah |
author_facet |
Wan Hanafi, Wan Nurhayati Abu Bakar, Nur Syahirah |
author_sort |
Wan Hanafi, Wan Nurhayati |
title |
Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar |
title_short |
Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar |
title_full |
Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar |
title_fullStr |
Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar |
title_full_unstemmed |
Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar |
title_sort |
application of whatman fta card method in oil palm dna extraction and pcr analysis / wan nurhayati wan hanafi and nur syahirah abu bakar |
publisher |
Penerbit UiTM (UiTM Press) |
publishDate |
2019 |
url |
http://ir.uitm.edu.my/id/eprint/28261/1/28261.pdf http://ir.uitm.edu.my/id/eprint/28261/ https://srj.uitm.edu.my/ https://doi.org/10.24191/srj.v16i2.6306 |
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1685650388933410816 |