Optimization of immobilization conditions for protease extracted from torpedo scad (megalaspis cordyla) viscera using response surface methodology (RSM) / Normah Ismail and Syafiqah Naziha

• Main Authors: Ismail, Normah, Naziha, Syafiqah
• Format: Article
• Language: English
• Published: Universiti Teknologi MARA 2019
• Subjects: Physical and theoretical chemistry; Extraction (Chemistry)
• Online Access: https://ir.uitm.edu.my/id/eprint/6106/1/6106.pdf; https://ir.uitm.edu.my/id/eprint/6106/; https://scilett-fsg.uitm.edu.my/
• Institution: Universiti Teknologi Mara

Protease was extracted from the viscera of torpedo scad fish (Megalspis cordyla) to obtain the crude extract which was then partially purified in 70% ammonium sulphate. The collected precipitate was dialysed and subsequently immobilized in sodium alginate and calcium chloride solution. The optimum c...