Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad

Lipolytic thermophilic bacteria was successfully isolated from Hulu Langat hot spring water. Molecular biological technique based on 16SRNA analysis revealed that the isolated bacteria species was Bacillus subtilis. Selective isolation of bacteria from hotspring water is performed using Rhodamine-B...

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Main Author: Mohamad, Ummul Hanan
Format: Thesis
Language:English
Published: 2009
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Online Access:https://ir.uitm.edu.my/id/eprint/70121/1/70121.pdf
https://ir.uitm.edu.my/id/eprint/70121/
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Institution: Universiti Teknologi Mara
Language: English
id my.uitm.ir.70121
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spelling my.uitm.ir.701212023-05-21T14:47:32Z https://ir.uitm.edu.my/id/eprint/70121/ Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad Mohamad, Ummul Hanan Environmental protection Lipolytic thermophilic bacteria was successfully isolated from Hulu Langat hot spring water. Molecular biological technique based on 16SRNA analysis revealed that the isolated bacteria species was Bacillus subtilis. Selective isolation of bacteria from hotspring water is performed using Rhodamine-B Tween 80 agar in which lipase producer is identified based on presence of orange fluorescence halos around colonies when plate is irradiated with UV light. Further characterisation of isolated bacteria is performed based on the colony characteristics on media as well as Gram staining. Bacillus subtilis isolated from hotspring appeared as clear looking colony with reddish tint. It was irregular in shaped, flat, mucoid and big. It was also motile and catalase positive. Under Gram staining, it was arranged in chains and was big long rods. The isolated lipase producer grew best at 55°C at alkaline pH of 8.1. 2009 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/70121/1/70121.pdf Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad. (2009) Degree thesis, thesis, Universiti Teknologi MARA (UiTM).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Environmental protection
spellingShingle Environmental protection
Mohamad, Ummul Hanan
Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
description Lipolytic thermophilic bacteria was successfully isolated from Hulu Langat hot spring water. Molecular biological technique based on 16SRNA analysis revealed that the isolated bacteria species was Bacillus subtilis. Selective isolation of bacteria from hotspring water is performed using Rhodamine-B Tween 80 agar in which lipase producer is identified based on presence of orange fluorescence halos around colonies when plate is irradiated with UV light. Further characterisation of isolated bacteria is performed based on the colony characteristics on media as well as Gram staining. Bacillus subtilis isolated from hotspring appeared as clear looking colony with reddish tint. It was irregular in shaped, flat, mucoid and big. It was also motile and catalase positive. Under Gram staining, it was arranged in chains and was big long rods. The isolated lipase producer grew best at 55°C at alkaline pH of 8.1.
format Thesis
author Mohamad, Ummul Hanan
author_facet Mohamad, Ummul Hanan
author_sort Mohamad, Ummul Hanan
title Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
title_short Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
title_full Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
title_fullStr Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
title_full_unstemmed Screening and identification of thermophilic microorganisms that produce extracellular lipase from Hulu Langat Hot Spring / Ummul Hanan Mohamad
title_sort screening and identification of thermophilic microorganisms that produce extracellular lipase from hulu langat hot spring / ummul hanan mohamad
publishDate 2009
url https://ir.uitm.edu.my/id/eprint/70121/1/70121.pdf
https://ir.uitm.edu.my/id/eprint/70121/
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