Enhanced production of pinostrobin content and adventitious root biomass in Boesenbergia rotunda / Nur Salsabeela Mohd Rahim

• Main Author: Mohd Rahim, Nur Salsabeela
• Format: Thesis
• Language: English
• Published: 2016
• Subjects: Nutrition. Plant food. Assimilation of nitrogen, etc.
• Online Access: https://ir.uitm.edu.my/id/eprint/98973/1/98973.pdf; https://ir.uitm.edu.my/id/eprint/98973/
• Institution: Universiti Teknologi Mara

Currently, the market demand for high value plant based-ingredient for pharmaceuticals, food supplement, and cosmetic products are increasing. However, the amount and quality of bioactive compounds in field-grown plants is limited for industrial business because it is greatly influenced by environmental factors and harvesting time. Therefore, tissue culture or in vitro culture technique is considered as the most popular alternative to produce a stable and large amount of biomass and bioactive compounds. Adventitious root culture was chosen for Boesenbergia rotunda species because of it fast growth. To develop the initiation protocol of adventitious root culture of B. rotunda, the buds were surface sterilized using different concentrations and durations of treatment with commercial bleach (Clorox®). T3 was found to be the best surface sterilization protocol of B. rotunda by producing high number of noncontaminated and survived explants. The effect of MS strength, sucrose concentration, and inoculum weight were tested to enhance the biomass and pinostrobin content in adventitious root culture of B. rotunda. Result obtained showed that the biomass of adventitious roots were increased in 0.5 MS strength, 5% (w/v) sucrose concentration and 1.5 g FW inoculum weight, whereas the accumulation of pinostrobin content was enhanced in 0.5 MS strength, 5% (w/v) sucrose concentration and 1.0 g FW inoculum weight, respectively. These results showed that the type of culture conditions had strongly affected the production of in vitro adventitious roots biomass and pinostrobin content of B. rotunda.