Development of a PCR assay for the detectiion of nifH and nifD genes in indigenous photosynthetic bacteria

• Main Authors: Tan, Jee Weng, Thong, Kwai Lin, Arumugam, Nirmala Devi, Cheah, Wai Lian, Lai, Yih Wei, Chua, Kek Heng, Rahim, Raha Abdul, Vikineswary, Sabaratnam
• Format: Article
• Language: English
• Published: Elsevier 2009
• Subjects: Q Science (General); QH Natural history; QR Microbiology; R Medicine
• Online Access: http://eprints.um.edu.my/10356/1/2009_Development_of_a_PCR_assay_for_the_detectiion_of_nifH_and_nifD_genes_in_indigenous_photosynthetic_bacteria..pdf; http://eprints.um.edu.my/10356/; https://doi.org/10.1016/j.ijhydene.2009.04.029
• Institution: Universiti Malaya

Molybdenum (Mo) nitrogenases consist of two components: dinitrogenase reductase (encoded by nifH) and the dinitrogenase or MoFe protein (encoded by nifDK). Nitrogenase enzyme of photosynthetic bacteria is responsible for hydrogen production. Therefore, primers were designed for the nitrogenase gene...