Dysregulation of miR-31 and miR-375 expression is associated with clinical outcomes in oral carcinoma

• Main Authors: Siow, M.Y., Ng, L.P.K., Chong, V.K.V., Jamaludin, M., Abraham, M.T., Rahman, Z.A.A., Kallarakkal, T.G., Yang, Y.H., Cheong, S.C., Zain, R.B.
• Format: Article
• Language: English
• Published: Blackwell Publishing 2014
• Subjects: RK Dentistry
• Online Access: http://eprints.um.edu.my/10479/1/Dysregulation_of_miR-31_and_miR-375_expression_is_associated_with_clinical_outcomes_in_oral_carcinoma.pdf; http://eprints.um.edu.my/10479/
• Institution: Universiti Malaya

Objectives To identify differentially expressed miRNA between oral squamous cell carcinoma (OSCC) and non-cancer (NC) and to associate these with clinico-pathological parameters. Materials and methods miRNA microarray profiling was utilized to obtain the expression profile of miRNAs in four OSCC and four NC samples. The expression of miR-31 and miR-375 was further validated in 26 OSCC and three NC samples using real-time-PCR. The association between miRNA expression and clinico-pathological parameters was tested by univariate and multivariate analyses. Results Microarray profiling demonstrated that 15 and four miRNAs were up-regulated and down-regulated, respectively, in OSCC as compared with NC. miR-31 and miR-375 were validated as up- and down-regulated miRNAs, respectively. In univariate analyses, expression of miR-31 was significantly elevated in early stage, tumours with no metastatic nodes and those from the buccal mucosa. By contrast, low miR-375 expression was significantly associated with late stage disease, larger tumour size and the non-cohesive type of pattern of invasion in OSCC. The association between miR-31 expression with tumour staging and site and miR-375 with tumour staging remained significant in multivariate analyses. Conclusions This study has identified 19 miRNAs significantly associated with OSCC, and expressions of miR-31 and miR-375 were significantly related with clinico-pathological parameters suggesting they could be important in driving oral tumourigenesis.