Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia

The emergence of Escherichia coli that produce extended spectrum beta-lactamases (ESBLs) and are multidrug resistant (MDR) poses antibiotic management problems. Forty-seven E. coli isolates from various public hospitals in Malaysia were studied. All isolates were sensitive to imipenem whereas 36 wer...

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Main Authors: Lim, K., Yasin, R., Yeo, C., Puthucheary, S., Thong, Kwai Lin
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2009
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Online Access:http://eprints.um.edu.my/1133/1/Characterization_of_Multidrug_Resistant_ESBL-Producing_Escherichia_coli_Isolates_from_Hospitals_in_Malaysia.pdf
http://eprints.um.edu.my/1133/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2721974/
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spelling my.um.eprints.11332018-10-15T04:20:29Z http://eprints.um.edu.my/1133/ Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia Lim, K. Yasin, R. Yeo, C. Puthucheary, S. Thong, Kwai Lin R Medicine The emergence of Escherichia coli that produce extended spectrum beta-lactamases (ESBLs) and are multidrug resistant (MDR) poses antibiotic management problems. Forty-seven E. coli isolates from various public hospitals in Malaysia were studied. All isolates were sensitive to imipenem whereas 36 were MDR (resistant to 2 or more classes of antibiotics). PCR detection using gene-specific primers showed that 87.5% of the ESBL-producing E. coli harbored the blaTEM gene. Other ESBL-encoding genes detected were blaOXA, blaSHV, and blaCTX-M. Integron-encoded integrases were detected in 55.3% of isolates, with class 1 integron-encoded intI1 integrase being the majority. Amplification and sequence analysis of the 5'CS region of the integrons showed known antibiotic resistance-encoding gene cassettes of various sizes that were inserted within the respective integrons. Conjugation and transformation experiments indicated that some of the antibiotic resistance genes were likely plasmid-encoded and transmissible. All 47 isolates were subtyped by PFGE and PCR-based fingerprinting using random amplified polymorphic DNA (RAPD), repetitive extragenic palindromes (REPs), and enterobacterial repetitive intergenic consensus (ERIC). These isolates were very diverse and heterogeneous. PFGE, ERIC, and REP-PCR methods were more discriminative than RAPD in subtyping the E. coli isolates. Hindawi Publishing Corporation 2009 Article PeerReviewed application/pdf en http://eprints.um.edu.my/1133/1/Characterization_of_Multidrug_Resistant_ESBL-Producing_Escherichia_coli_Isolates_from_Hospitals_in_Malaysia.pdf Lim, K. and Yasin, R. and Yeo, C. and Puthucheary, S. and Thong, Kwai Lin (2009) Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia. Journal of Biomedicine and Biotechnology, 2009. p. 165637. ISSN 1110-7243 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2721974/ 19672454
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Lim, K.
Yasin, R.
Yeo, C.
Puthucheary, S.
Thong, Kwai Lin
Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia
description The emergence of Escherichia coli that produce extended spectrum beta-lactamases (ESBLs) and are multidrug resistant (MDR) poses antibiotic management problems. Forty-seven E. coli isolates from various public hospitals in Malaysia were studied. All isolates were sensitive to imipenem whereas 36 were MDR (resistant to 2 or more classes of antibiotics). PCR detection using gene-specific primers showed that 87.5% of the ESBL-producing E. coli harbored the blaTEM gene. Other ESBL-encoding genes detected were blaOXA, blaSHV, and blaCTX-M. Integron-encoded integrases were detected in 55.3% of isolates, with class 1 integron-encoded intI1 integrase being the majority. Amplification and sequence analysis of the 5'CS region of the integrons showed known antibiotic resistance-encoding gene cassettes of various sizes that were inserted within the respective integrons. Conjugation and transformation experiments indicated that some of the antibiotic resistance genes were likely plasmid-encoded and transmissible. All 47 isolates were subtyped by PFGE and PCR-based fingerprinting using random amplified polymorphic DNA (RAPD), repetitive extragenic palindromes (REPs), and enterobacterial repetitive intergenic consensus (ERIC). These isolates were very diverse and heterogeneous. PFGE, ERIC, and REP-PCR methods were more discriminative than RAPD in subtyping the E. coli isolates.
format Article
author Lim, K.
Yasin, R.
Yeo, C.
Puthucheary, S.
Thong, Kwai Lin
author_facet Lim, K.
Yasin, R.
Yeo, C.
Puthucheary, S.
Thong, Kwai Lin
author_sort Lim, K.
title Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia
title_short Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia
title_full Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia
title_fullStr Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia
title_full_unstemmed Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia
title_sort characterization of multidrug resistant esbl-producing escherichia coli isolates from hospitals in malaysia
publisher Hindawi Publishing Corporation
publishDate 2009
url http://eprints.um.edu.my/1133/1/Characterization_of_Multidrug_Resistant_ESBL-Producing_Escherichia_coli_Isolates_from_Hospitals_in_Malaysia.pdf
http://eprints.um.edu.my/1133/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2721974/
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