Immunoblot analysis of antigens from Parastrongylus cantonensis, P. costaricensis and P. malaysiensis using serum antibodies against P. cantonensis

• Main Authors: Eamsobhana, P., Yong, Hoi Sen, Ambu, S.
• Format: Article
• Published: Japanese Society of Tropical Medicine 2000
• Subjects: Q Science (General); QH Natural history
• Online Access: http://eprints.um.edu.my/13398/; https://www.jstage.jst.go.jp/article/tmh1973/28/2/28_2_115/_pdf
• Institution: Universiti Malaya

Parastrongylus cantonensis, P. costaricensis and P. malaysiensis adult worm antigens were tested for cross-reactivities in immunoblots using sera from patients with parasitologically confirmed parastrongyliasis. Sera from P. cantonensis-infected patients with a high specificity for the 31-kDa diagnostic P. cantonensis antigen cross-reacted with a corresponding component found in P. costaricensis and P. malaysiensis. The immune sera consistently recognized a prominent immunogenic band with a MW of 31 kDa against the antigens prepared from the 3 Parastrongylus species. Sera obtained from patients infected with Gnathostoma spinigerum and normal healthy control sera did not react with the 31-kDa protein from the 3 species of Parastrongylus. The cross-reactivity observed in the immunoblots performed on serum samples from patients with P. cantonensis infection indicates that the 3 Parastrongylus species share the specific 31-kDa antigen. This protein may be used for the immunodiagnosis of human infection with P. costaricensis and P. malaysiensis.