Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia

BACKGROUND: The objectives of the present study were to determine the antimicrobial resistance, virulotypes and genetic diversity of Yersinia enterocolitica isolated from uncooked porcine food and live pigs in Malaysia. RESULTS: Thirty-two non-repeat Y. enterocolitica strains of three bioserotypes...

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Main Authors: Thong, Kwai Lin, Tan, Lai Kuan, Ooi, Peck Toung
Format: Article
Language:English
Published: Wiley 2017
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Online Access:http://eprints.um.edu.my/18224/1/Genetic_diversity%2C_virulotyping.pdf
http://eprints.um.edu.my/18224/
https://doi.org/10.1002/jsfa.8442
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spelling my.um.eprints.182242019-03-21T06:32:27Z http://eprints.um.edu.my/18224/ Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia Thong, Kwai Lin Tan, Lai Kuan Ooi, Peck Toung Q Science (General) QH Natural history BACKGROUND: The objectives of the present study were to determine the antimicrobial resistance, virulotypes and genetic diversity of Yersinia enterocolitica isolated from uncooked porcine food and live pigs in Malaysia. RESULTS: Thirty-two non-repeat Y. enterocolitica strains of three bioserotypes (3 variant/O:3, n = 27; 1B/O:8, n = 3; 1A/O:5, n = 2) were analysed. Approximately 90% of strains were multidrug-resistant with a multiple antibiotic resistance index < 0.2 and the majority of the strains were resistant to nalidixic acid, clindamycin, ampicillin, ticarcillin, tetracycline and amoxicillin. Yersinia enterocolitica could be distinguished distinctly into three clusters by pulsed-field gel electrophoresis, with each belonging to a particular bioserotype. Strains of 3 variant/O:3 were more heterogeneous than others. Eleven of the 15 virulence genes tested (hreP, virF, rfbC, myfA, sat, inv, ail, ymoA, ystA, tccC, yadA) and pYV virulence plasmid were present in all the bioserotpe 3 variant/03 strains. CONCLUSION: The occurrence of virulent strains of Y. enterocolitica in pigs and porcine products reiterated that pigs are important reservoirs for Y. enterocolitica. The increasing trend of multidrug resistant strains is a public health concern. This is the first report on the occurrence of potential pathogenic and resistant strains of Y. enterocolitica in pigs in Malaysia. Wiley 2017-05-24 Article PeerReviewed application/pdf en http://eprints.um.edu.my/18224/1/Genetic_diversity%2C_virulotyping.pdf Thong, Kwai Lin and Tan, Lai Kuan and Ooi, Peck Toung (2017) Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia. Journal of the Science of Food and Agriculture, 98 (1). pp. 87-95. ISSN 0022-5142 https://doi.org/10.1002/jsfa.8442 doi:10.1002/jsfa.8442
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Thong, Kwai Lin
Tan, Lai Kuan
Ooi, Peck Toung
Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia
description BACKGROUND: The objectives of the present study were to determine the antimicrobial resistance, virulotypes and genetic diversity of Yersinia enterocolitica isolated from uncooked porcine food and live pigs in Malaysia. RESULTS: Thirty-two non-repeat Y. enterocolitica strains of three bioserotypes (3 variant/O:3, n = 27; 1B/O:8, n = 3; 1A/O:5, n = 2) were analysed. Approximately 90% of strains were multidrug-resistant with a multiple antibiotic resistance index < 0.2 and the majority of the strains were resistant to nalidixic acid, clindamycin, ampicillin, ticarcillin, tetracycline and amoxicillin. Yersinia enterocolitica could be distinguished distinctly into three clusters by pulsed-field gel electrophoresis, with each belonging to a particular bioserotype. Strains of 3 variant/O:3 were more heterogeneous than others. Eleven of the 15 virulence genes tested (hreP, virF, rfbC, myfA, sat, inv, ail, ymoA, ystA, tccC, yadA) and pYV virulence plasmid were present in all the bioserotpe 3 variant/03 strains. CONCLUSION: The occurrence of virulent strains of Y. enterocolitica in pigs and porcine products reiterated that pigs are important reservoirs for Y. enterocolitica. The increasing trend of multidrug resistant strains is a public health concern. This is the first report on the occurrence of potential pathogenic and resistant strains of Y. enterocolitica in pigs in Malaysia.
format Article
author Thong, Kwai Lin
Tan, Lai Kuan
Ooi, Peck Toung
author_facet Thong, Kwai Lin
Tan, Lai Kuan
Ooi, Peck Toung
author_sort Thong, Kwai Lin
title Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia
title_short Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia
title_full Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia
title_fullStr Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia
title_full_unstemmed Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia
title_sort genetic diversity, virulotyping and antimicrobial resistance susceptibility of yersinia enterocolitica isolated from pigs and porcine products in malaysia
publisher Wiley
publishDate 2017
url http://eprints.um.edu.my/18224/1/Genetic_diversity%2C_virulotyping.pdf
http://eprints.um.edu.my/18224/
https://doi.org/10.1002/jsfa.8442
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