Effect of hesperidin on the temporal regulation of redox homeostasis in clock mutant (Cryb) of Drosophila melanogaster

• Main Authors: Arumugam, Manjula, Jayapalan, Jaime Jacqueline, Abdul-Rahman, Puteri Shafinaz, Hashim, Onn Haji, Subramanian, Perumal
• Format: Article
• Published: Taylor & Francis 2018
• Subjects: QD Chemistry; R Medicine
• Online Access: http://eprints.um.edu.my/22449/; https://doi.org/10.1080/09291016.2017.1333567
• Institution: Universiti Malaya

Disorganized redox homeostasis is a main factor causing a number of diseases and it is imperative to comprehend the orchestration of circadian clock under oxidative stress in the organism, Drosophila melanogaster. This investigation analyses the influence of hesperidin on the circadian rhythms of lipid peroxidation products and antioxidants during rotenone-stimulated oxidative stress in fruit fly. The characteristics of rhythms of thiobarbituric acid reactive substances (TBARS), antioxidants (superoxide dismutase (SOD) and catalase (CAT)) were noticeably decreased in rotenone administered flies. Supplementation of hesperidin to rotenone-treated flies increased the mesor and modulated the amplitudes of antioxidants and conspicuously decreased the mesor values of TBARS. In addition, delays in acrophase in rotenone-induced flies were reversed by hesperidin treatment. Thus, treatment of hesperidin caused normalization of the altered rhythms. Disorganization of 24 h rhythms in markers of redox homeostasis was observed during rotenone treatment and the impairment is severe in circadian clock mutant (Cryb) flies. Reversibility of rhythms was prominent subsequent to hesperidin treatment in wild-type flies than (Cryb) flies. These observations denote a role of circadian clock in redox homeostasis and the use of Drosophila model in screening putative antioxidative phytomedicines prior to their usage in mammalian systems.