Serum from Nipah virus patients recognises recombinant viral proteins produced in Escherichia coli

The genes for Nipah virus (NiV) proteins were amplified from viral RNA, cloned into the plasmid pTriEx-3 Hygro, expressed, and purified using immobilized metal affinity chromatography. The recombinant N, F, and G NiV proteins (rNiV-N, rNiV-F, and rNiV-G), were successfully expressed in Escherichia c...

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Bibliographic Details
Main Authors: Tiong, Vunjia, Lam, Chui Wan, Phoon, Wai Hong, AbuBakar, Sazaly, Chang, Li Yen
Format: Article
Published: Institute of Infectious Diseases 2017
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Online Access:http://eprints.um.edu.my/22882/
https://doi.org/10.7883/yoken.JJID.2015.501
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Institution: Universiti Malaya