High-quality RNA isolation from pigment-rich Dendrobium flowers

• Main Authors: Khairul Anuar, Muhammad Asyraf, Mazumdar, Purabi, Lau, Su Ee, Tan, Tian Tian, Harikrishna, Jennifer Ann
• Format: Article
• Published: Springer Verlag (Germany) 2019
• Subjects: QH Natural history; S Agriculture (General)
• Online Access: http://eprints.um.edu.my/24222/; https://doi.org/10.1007/s13205-019-1898-y
• Institution: Universiti Malaya

Isolation of high-quality RNA from Dendrobium flowers is challenging because of the high levels of pigment, polysaccharides, and polyphenols. In the present study, an efficient CTAB method for RNA extraction from the pigment-rich flowers of Dendrobium was optimised. The optimised method yielded high...