Bacterial diversity of decomposing oil palm empty fruit bunches based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments
The microbial community that inhabit the thermophilic stage of composting process consist of complex thermophile communities that interact with each other to biodegrade lignocellulosic materials. The diversity of this community during oil palm empty fruit bunches (OPEFB) composting was investigated...
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2007
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| Online Access: | http://eprints.um.edu.my/25111/1/Bacterial%20diversity%20of%20decomposing%20oil%20palm%20empty%20fruit%20bunches%20based%20on%20PCR%20and%20denaturing%20gradient%20gel%20electrophoresis%20of%2016S%20rRNA%20gene%20fragments.pdf http://eprints.um.edu.my/25111/ https://www.mymicro.org/ |
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my.um.eprints.251112020-07-17T01:06:19Z http://eprints.um.edu.my/25111/ Bacterial diversity of decomposing oil palm empty fruit bunches based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments Goh, C.M. Liew, P.W.Y. Jong, B.C. Thong, Kwai Lin Q Science (General) QR Microbiology The microbial community that inhabit the thermophilic stage of composting process consist of complex thermophile communities that interact with each other to biodegrade lignocellulosic materials. The diversity of this community during oil palm empty fruit bunches (OPEFB) composting was investigated using PCR and denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. Compost samples were collected during the thermophilic phase with a temperature of between 60°C-62°C. Based on DGGE profile of 16S rDNA amplicons, four discrete bands were observed. Sequence analysis of the excised bands showed that sequences of these bands are closely related to those found in uncultured bacteria from other compost environments. The results suggest that the composting process of OPEFB during the thermophilic phase were dominated by yet-uncultured bacteria species. 2007 Conference or Workshop Item PeerReviewed text en http://eprints.um.edu.my/25111/1/Bacterial%20diversity%20of%20decomposing%20oil%20palm%20empty%20fruit%20bunches%20based%20on%20PCR%20and%20denaturing%20gradient%20gel%20electrophoresis%20of%2016S%20rRNA%20gene%20fragments.pdf Goh, C.M. and Liew, P.W.Y. and Jong, B.C. and Thong, Kwai Lin (2007) Bacterial diversity of decomposing oil palm empty fruit bunches based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments. In: 29th Symposium of the Malaysian Society for Microbiology, 24-26 November 2007, Kuala Terengganu, Terengganu, Malaysia. (Unpublished) https://www.mymicro.org/ |
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Q Science (General) QR Microbiology Goh, C.M. Liew, P.W.Y. Jong, B.C. Thong, Kwai Lin Bacterial diversity of decomposing oil palm empty fruit bunches based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments |
| description |
The microbial community that inhabit the thermophilic stage of composting process consist of complex thermophile communities that interact with each other to biodegrade lignocellulosic materials. The diversity of this community during oil palm empty fruit bunches (OPEFB) composting was investigated using PCR and denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. Compost samples were collected during the thermophilic phase with a temperature of between 60°C-62°C. Based on DGGE profile of 16S rDNA amplicons, four discrete bands were observed. Sequence analysis of the excised bands showed that sequences of these bands are closely related to those found in uncultured bacteria from other compost environments. The results suggest that the composting process of OPEFB during the thermophilic phase were dominated by yet-uncultured bacteria species. |
| format |
Conference or Workshop Item |
| author |
Goh, C.M. Liew, P.W.Y. Jong, B.C. Thong, Kwai Lin |
| author_facet |
Goh, C.M. Liew, P.W.Y. Jong, B.C. Thong, Kwai Lin |
| author_sort |
Goh, C.M. |
| title |
Bacterial diversity of decomposing oil palm empty fruit bunches based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments |
| title_short |
Bacterial diversity of decomposing oil palm empty fruit bunches based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments |
| title_full |
Bacterial diversity of decomposing oil palm empty fruit bunches based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments |
| title_fullStr |
Bacterial diversity of decomposing oil palm empty fruit bunches based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments |
| title_full_unstemmed |
Bacterial diversity of decomposing oil palm empty fruit bunches based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments |
| title_sort |
bacterial diversity of decomposing oil palm empty fruit bunches based on pcr and denaturing gradient gel electrophoresis of 16s rrna gene fragments |
| publishDate |
2007 |
| url |
http://eprints.um.edu.my/25111/1/Bacterial%20diversity%20of%20decomposing%20oil%20palm%20empty%20fruit%20bunches%20based%20on%20PCR%20and%20denaturing%20gradient%20gel%20electrophoresis%20of%2016S%20rRNA%20gene%20fragments.pdf http://eprints.um.edu.my/25111/ https://www.mymicro.org/ |
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