Guanidine hydrochloride-induced denaturation of bovine serum albumin: A comparative study and analysis using different probes
The denaturation of bovine serum albumin (BSA) by guanidine hydrochloride (GdnHCl) showed a single-step, two-state transition, when monitored by different probes such as intrinsic fluorescence at 338 and 333 nm after excitation at 280 and 295 nm respectively, UV difference spectral signal at 288 nm,...
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Main Authors: | , , |
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Format: | Article |
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Faculty of Science, University of Malaya
2008
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Online Access: | http://eprints.um.edu.my/25651/ https://mjs.um.edu.my/article/view/7886 |
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Institution: | Universiti Malaya |