Synthesis of capsaicin loaded silver nanoparticles using green approach and its anti-bacterial activity against human pathogens

• Main Authors: Mahmood, Syed, Mei, Tan Siew, Yee, Wong Xi, Hilles, Ayah Rebhi, Alelwani, Walla, Bannunah, Azzah M
• Format: Article
• Published: 2021
• Subjects: R Medicine; RS Pharmacy and materia medica
• Online Access: http://eprints.um.edu.my/26210/; https://doi.org/10.1166/jbn.2021.3122
• Institution: Universiti Malaya

Nanotechnology is drawing attention nowadays due to its ability to regulate metals into nanosize, ultimately changing metal's physical, chemical, and IP:optical proper49.249.253ties. 194Silver O: nanoparWed, ticles22 Separe known2021 for06 their07:37potential impact as antimicrobial agents due to their inherent property penetrating the cell wall. The present study aimed to develop and statistically Copyright: American Scentifc Publishers optimise using a novel combination of capsaicin loaded silver nanoparticles (AgCNPs) as an effective anti-bacterial agent Delivered by Ingenta to treat psoriasis using a green approach. Ascorbic acid was used as a reducing agent to fabricate silver nanoparticles. The formulation parameters optimisation was conducted using Box-Behnken Design (3x3 factorial design). The loading of capsaicin was confirmed by attenuated total reflectance-fourier transform infrared spectroscopy. Energy-dispersive X-ray spectroscopy-scanning electron microscopy (EDX-SEM) confirmed the existence of silver; net-like structure revealed in SEM and high-resolution transmission electron microscopy further confirmed the nano size of the formulation. Differential scanning calorimetry and X-ray diffraction demonstrated the capsaicin transformed into amorphous after encapsulated. An in-vitro microbial study showed that the 0.10 M formulation of AgCNPs exerted potent anti-bacterial activity, which can be considered an alternative anti-bacterial agent. It also displayed that the zone of inhibition was significantly high in gram-negative bacteria (E. coli) than gram-positive bacteria (S. aureus). Green synthesised AgCNPs showed highly significant anti-bacterial activity, which indicates that this formulation can be very promising for treating psoriasis.