In vitroandin silicoanticancer evaluation of a medicinal mushroom, Ganoderma neo-japonicumImazeki, against human colonic carcinoma cells

• Main Authors: Lau, Meng-Fei, Chua, Kek-Heng, Sabaratnam, Vikineswary, Kuppusamy, Umah Rani
• Format: Article
• Published: Wiley 2021
• Subjects: QD Chemistry; QH301 Biology; R Medicine (General)
• Online Access: http://eprints.um.edu.my/26555/
• Institution: Universiti Malaya

Ganoderma neo-japonicumis a well-known medicinal mushroom in Asian countries. However, scientific validations on its curative activities are confined to cirrhosis and diabetes. In this study, the anticancer properties ofG. neo-japonicumwere evaluated using cellular and computational models. The ethanolic extract (EtOH) with a promising inhibitory effect was fractionated into four different fractions: hexane (Hex), chloroform (Chl), butanol (Btn), and aqueous (Aq). The active fractions were then subjected to cell apoptosis assessment and phytochemical profiling. Molecular docking was conducted to elucidate the affinity of selected constituents towards antiapoptotic Bcl-2 protein. The butanol fraction showed the highest antioxidant activities as well as total phenolic content. Both hexane and chloroform fractions exerted a potent cytotoxic effect on colonic carcinoma cells through the induction of apoptosis. Phytochemical analysis revealed that the chloroform fraction is terpenoid enriched whereas the hexane fraction comprises predominantly sterol constituents. Stellasterol and 1,25-dihydroxyvitamin D3 3-glycoside were demonstrated to have a high affinity towards Bcl-2 protein. Overall,G. neo-japonicumcan be considered as a compelling therapeutic candidate for cancer treatment.