Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia
The cytochrome oxidase subunit I (COXI) gene sequences of three recent (20072008) clinical Plasmodium knowlesi isolates from Klang Valley, peninsular Malaysia, were determined and compared with those of older (1960's) peninsular Malaysia, recent isolates from Sarawak (on Borneo Island), and an...
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Malaysian Society of Parasitology and Tropical Medicine
2011
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my.um.eprints.27222019-11-14T07:58:10Z http://eprints.um.edu.my/2722/ Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia Fong, Mun Yik Lau, Yee Ling Chin, Lit Chien Al-Mekhlafi, A.M.Q. R Medicine The cytochrome oxidase subunit I (COXI) gene sequences of three recent (20072008) clinical Plasmodium knowlesi isolates from Klang Valley, peninsular Malaysia, were determined and compared with those of older (1960's) peninsular Malaysia, recent isolates from Sarawak (on Borneo Island), and an isolate from Thailand. Multiple alignment of the sequences showed that the three clinical isolates were more similar to the older peninsular Malaysia isolates than to those from Sarawak and Thailand. Phylogenetic tree based on the COXI sequences revealed three distinct clusters of P. knowlesi. The first cluster consisted of isolates from peninsular Malaysia, the second consisted of Sarawak isolates and the third composed of the Thailand isolate. The findings of this study highlight the usefulness of mitochondrial COXI gene as a suitable marker for phylogeographic studies of P. knowlesi. Malaysian Society of Parasitology and Tropical Medicine 2011 Article PeerReviewed application/pdf en http://eprints.um.edu.my/2722/1/457_-_463_Mun_Yik_Fong.pdf Fong, Mun Yik and Lau, Yee Ling and Chin, Lit Chien and Al-Mekhlafi, A.M.Q. (2011) Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia. Tropical Biomedicine, 28 (2). pp. 457-463. ISSN 0127-5720 http://www.msptm.org/files/457_-_463_Mun_Yik_Fong.pdf |
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R Medicine Fong, Mun Yik Lau, Yee Ling Chin, Lit Chien Al-Mekhlafi, A.M.Q. Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia |
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The cytochrome oxidase subunit I (COXI) gene sequences of three recent (20072008) clinical Plasmodium knowlesi isolates from Klang Valley, peninsular Malaysia, were determined and compared with those of older (1960's) peninsular Malaysia, recent isolates from Sarawak (on Borneo Island), and an isolate from Thailand. Multiple alignment of the sequences showed that the three clinical isolates were more similar to the older peninsular Malaysia isolates than to those from Sarawak and Thailand. Phylogenetic tree based on the COXI sequences revealed three distinct clusters of P. knowlesi. The first cluster consisted of isolates from peninsular Malaysia, the second consisted of Sarawak isolates and the third composed of the Thailand isolate. The findings of this study highlight the usefulness of mitochondrial COXI gene as a suitable marker for phylogeographic studies of P. knowlesi. |
format |
Article |
author |
Fong, Mun Yik Lau, Yee Ling Chin, Lit Chien Al-Mekhlafi, A.M.Q. |
author_facet |
Fong, Mun Yik Lau, Yee Ling Chin, Lit Chien Al-Mekhlafi, A.M.Q. |
author_sort |
Fong, Mun Yik |
title |
Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia |
title_short |
Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia |
title_full |
Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia |
title_fullStr |
Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia |
title_full_unstemmed |
Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia |
title_sort |
sequence analysis on the mitochondrial coxi gene of recent clinical isolates of plasmodium knowlesi in klang valley, peninsular malaysia |
publisher |
Malaysian Society of Parasitology and Tropical Medicine |
publishDate |
2011 |
url |
http://eprints.um.edu.my/2722/1/457_-_463_Mun_Yik_Fong.pdf http://eprints.um.edu.my/2722/ http://www.msptm.org/files/457_-_463_Mun_Yik_Fong.pdf |
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