High diversity of cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene
Background: Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive pati...
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my.um.eprints.33392017-07-05T01:33:22Z http://eprints.um.edu.my/3339/ High diversity of cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene Iqbal, A. Lim, Y.A.L. Surin, J. Sim, B L.H. R Medicine Background: Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive patients. Methodology/Principal Findings: In this study, 346 faecal samples collected from Malaysian HIV positive patients were genetically analysed via PCR targeting the 60 kDa glycoprotein (gp60) gene. Eighteen (5.2 of 346) isolates were determined as Cryptosporidium positive with 72.2 (of 18) identified as Cryptosporidium parvum whilst 27.7 as Cryptosporidium hominis. Further gp60 analysis revealed C. parvum belonging to subgenotypes IIaA13G1R1 (2 isolates), IIaA13G2R1 (2 isolates), IIaA14G2R1 (3 isolates), IIaA15G2R1 (5 isolates) and IIdA15G1R1 (1 isolate). C. hominis was represented by subgenotypes IaA14R1 (2 isolates), IaA18R1 (1 isolate) and IbA10G2R2 (2 isolates). Conclusions/Significance: These findings highlighted the presence of high diversity of Cryptosporidium subgenotypes among Malaysian HIV infected individuals. The predominance of the C. parvum subgenotypes signified the possibility of zoonotic as well as anthroponotic transmissions of cryptosporidiosis in HIV infected individuals. Public Library of Science 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3339/1/High_Diversity_of_Cryptosporidium_Subgenotypes.pdf Iqbal, A. and Lim, Y.A.L. and Surin, J. and Sim, B L.H. (2012) High diversity of cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene. PLoS ONE, 7 (2). ISSN 1932-6203 |
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R Medicine Iqbal, A. Lim, Y.A.L. Surin, J. Sim, B L.H. High diversity of cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
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Background: Currently, there is a lack of vital information in the genetic makeup of Cryptosporidium especially in developing countries. The present study aimed at determining the genotypes and subgenotypes of Cryptosporidium in hospitalized Malaysian human immunodeficiency virus (HIV) positive patients. Methodology/Principal Findings: In this study, 346 faecal samples collected from Malaysian HIV positive patients were genetically analysed via PCR targeting the 60 kDa glycoprotein (gp60) gene. Eighteen (5.2 of 346) isolates were determined as Cryptosporidium positive with 72.2 (of 18) identified as Cryptosporidium parvum whilst 27.7 as Cryptosporidium hominis. Further gp60 analysis revealed C. parvum belonging to subgenotypes IIaA13G1R1 (2 isolates), IIaA13G2R1 (2 isolates), IIaA14G2R1 (3 isolates), IIaA15G2R1 (5 isolates) and IIdA15G1R1 (1 isolate). C. hominis was represented by subgenotypes IaA14R1 (2 isolates), IaA18R1 (1 isolate) and IbA10G2R2 (2 isolates). Conclusions/Significance: These findings highlighted the presence of high diversity of Cryptosporidium subgenotypes among Malaysian HIV infected individuals. The predominance of the C. parvum subgenotypes signified the possibility of zoonotic as well as anthroponotic transmissions of cryptosporidiosis in HIV infected individuals. |
format |
Article |
author |
Iqbal, A. Lim, Y.A.L. Surin, J. Sim, B L.H. |
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Iqbal, A. Lim, Y.A.L. Surin, J. Sim, B L.H. |
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Iqbal, A. |
title |
High diversity of cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
title_short |
High diversity of cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
title_full |
High diversity of cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
title_fullStr |
High diversity of cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
title_full_unstemmed |
High diversity of cryptosporidium subgenotypes identified in Malaysian HIV/AIDS individuals targeting gp60 gene |
title_sort |
high diversity of cryptosporidium subgenotypes identified in malaysian hiv/aids individuals targeting gp60 gene |
publisher |
Public Library of Science |
publishDate |
2012 |
url |
http://eprints.um.edu.my/3339/1/High_Diversity_of_Cryptosporidium_Subgenotypes.pdf http://eprints.um.edu.my/3339/ |
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1643687095544315904 |