Comparative studies of the biological efficacies of Ag and Ag-MgO nanocomposite formed by the green synthesis route

The present study investigates the changes occurring to the physicochemical and biological properties of silver (Ag) nanoparticles (NPs) before and after conjugating with that of magnesium oxide (MgO). In that view, to form Ag NPs and Ag-MgO nanocomposite, we adopted a green synthesis route that mak...

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Main Authors: Sagadevan, Suresh, Lett, J. Anita, Murugan, Baranya, Fatimah, Is, Garg, Seema, Hossain, M. A. Motalib, Mohammad, Faruq, Al-Lohedan, Hamad A., Johan, Mohd Rafie
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Published: Elsevier 2022
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Online Access:http://eprints.um.edu.my/33644/
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spelling my.um.eprints.336442022-07-27T08:12:37Z http://eprints.um.edu.my/33644/ Comparative studies of the biological efficacies of Ag and Ag-MgO nanocomposite formed by the green synthesis route Sagadevan, Suresh Lett, J. Anita Murugan, Baranya Fatimah, Is Garg, Seema Hossain, M. A. Motalib Mohammad, Faruq Al-Lohedan, Hamad A. Johan, Mohd Rafie QD Chemistry The present study investigates the changes occurring to the physicochemical and biological properties of silver (Ag) nanoparticles (NPs) before and after conjugating with that of magnesium oxide (MgO). In that view, to form Ag NPs and Ag-MgO nanocomposite, we adopted a green synthesis route that makes use of Citrus paradisi (grapefruit red) extract as a reducing agent and the formed particles are thoroughly characterized for the crystallinity, surface bonding and functionality, morphology, composition, surface charges, etc. From the physicochemical analysis, the successful formation of Ag and Ag-MgO composites are confirmed from the UV-Vis and FTIR spectroscopy, while the TEM indicated the spherical morphology and DLS for the average particle size of 260 nm in solution. Further, on testing the biological efficacy, the Ag-MgO nanocomposite (up to 320 mu g/mL) was found to have efficient antibacterial and antifungal activities against Staphylococcus aureus and Candida albicans (respectively). Also, the studies of beta-galactosidase enzyme activity resulted that Ag's composite for-mation with MgO (Ag-MgO with IC50 of 27.6 mu g/mL) is not significantly enhancing the effectiveness as compared to pure Ag NPs (IC50 of 28.6 mu g/mL). Also, the in vitro cell viability studies indicated the anticancer activity of Ag-MgO nanocomposite in a dose-dependent manner when tested against the Hela cancer cells was in the con-centration range of 1-100 mu g/mL (24 h period). Based on the overall analysis, the easy making process by the green synthesis route and inexpensive Ag-MgO nanocomposite can serve as a superior antimicrobial and anti-cancer ingredient for the development of therapeutic products in the biomedical sector. Elsevier 2022-01 Article PeerReviewed Sagadevan, Suresh and Lett, J. Anita and Murugan, Baranya and Fatimah, Is and Garg, Seema and Hossain, M. A. Motalib and Mohammad, Faruq and Al-Lohedan, Hamad A. and Johan, Mohd Rafie (2022) Comparative studies of the biological efficacies of Ag and Ag-MgO nanocomposite formed by the green synthesis route. Inorganic Chemistry Communications, 135. ISSN 1387-7003, DOI https://doi.org/10.1016/j.inoche.2021.109082 <https://doi.org/10.1016/j.inoche.2021.109082>. 10.1016/j.inoche.2021.109082
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QD Chemistry
spellingShingle QD Chemistry
Sagadevan, Suresh
Lett, J. Anita
Murugan, Baranya
Fatimah, Is
Garg, Seema
Hossain, M. A. Motalib
Mohammad, Faruq
Al-Lohedan, Hamad A.
Johan, Mohd Rafie
Comparative studies of the biological efficacies of Ag and Ag-MgO nanocomposite formed by the green synthesis route
description The present study investigates the changes occurring to the physicochemical and biological properties of silver (Ag) nanoparticles (NPs) before and after conjugating with that of magnesium oxide (MgO). In that view, to form Ag NPs and Ag-MgO nanocomposite, we adopted a green synthesis route that makes use of Citrus paradisi (grapefruit red) extract as a reducing agent and the formed particles are thoroughly characterized for the crystallinity, surface bonding and functionality, morphology, composition, surface charges, etc. From the physicochemical analysis, the successful formation of Ag and Ag-MgO composites are confirmed from the UV-Vis and FTIR spectroscopy, while the TEM indicated the spherical morphology and DLS for the average particle size of 260 nm in solution. Further, on testing the biological efficacy, the Ag-MgO nanocomposite (up to 320 mu g/mL) was found to have efficient antibacterial and antifungal activities against Staphylococcus aureus and Candida albicans (respectively). Also, the studies of beta-galactosidase enzyme activity resulted that Ag's composite for-mation with MgO (Ag-MgO with IC50 of 27.6 mu g/mL) is not significantly enhancing the effectiveness as compared to pure Ag NPs (IC50 of 28.6 mu g/mL). Also, the in vitro cell viability studies indicated the anticancer activity of Ag-MgO nanocomposite in a dose-dependent manner when tested against the Hela cancer cells was in the con-centration range of 1-100 mu g/mL (24 h period). Based on the overall analysis, the easy making process by the green synthesis route and inexpensive Ag-MgO nanocomposite can serve as a superior antimicrobial and anti-cancer ingredient for the development of therapeutic products in the biomedical sector.
format Article
author Sagadevan, Suresh
Lett, J. Anita
Murugan, Baranya
Fatimah, Is
Garg, Seema
Hossain, M. A. Motalib
Mohammad, Faruq
Al-Lohedan, Hamad A.
Johan, Mohd Rafie
author_facet Sagadevan, Suresh
Lett, J. Anita
Murugan, Baranya
Fatimah, Is
Garg, Seema
Hossain, M. A. Motalib
Mohammad, Faruq
Al-Lohedan, Hamad A.
Johan, Mohd Rafie
author_sort Sagadevan, Suresh
title Comparative studies of the biological efficacies of Ag and Ag-MgO nanocomposite formed by the green synthesis route
title_short Comparative studies of the biological efficacies of Ag and Ag-MgO nanocomposite formed by the green synthesis route
title_full Comparative studies of the biological efficacies of Ag and Ag-MgO nanocomposite formed by the green synthesis route
title_fullStr Comparative studies of the biological efficacies of Ag and Ag-MgO nanocomposite formed by the green synthesis route
title_full_unstemmed Comparative studies of the biological efficacies of Ag and Ag-MgO nanocomposite formed by the green synthesis route
title_sort comparative studies of the biological efficacies of ag and ag-mgo nanocomposite formed by the green synthesis route
publisher Elsevier
publishDate 2022
url http://eprints.um.edu.my/33644/
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