Electrophoretic detection of salivary alpha-amylase activity

Fresh samples of human whole saliva containing approximately 20-40 micrograms protein were analyzed using SDS-polyacrylamide slab gel electrophoresis systems. More than 20 protein bands were revealed by Coomassie Brilliant Blue R 250 staining. Some of the protein bands were shown to be glycoprotein-...

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Main Authors: Rahim, Z.H., Yaacob, H.B.
Format: Article
Language:English
Published: 1992
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Online Access:http://eprints.um.edu.my/3750/1/Electrophoretic_detection_of_salivary_alpha-amylase_activity.pdf
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spelling my.um.eprints.37502012-10-10T01:52:17Z http://eprints.um.edu.my/3750/ Electrophoretic detection of salivary alpha-amylase activity Rahim, Z.H. Yaacob, H.B. RK Dentistry Fresh samples of human whole saliva containing approximately 20-40 micrograms protein were analyzed using SDS-polyacrylamide slab gel electrophoresis systems. More than 20 protein bands were revealed by Coomassie Brilliant Blue R 250 staining. Some of the protein bands were shown to be glycoprotein-positive with PAS (periodic acid-Schiff) reagent. The protein bands with alpha-Amylase activity appeared within a molecular weight range of 120,000-180,000, which is 2 to 2.8 times higher than the normal molecular weight reported for alpha-Amylase from parotid saliva, and showed positive staining with PAS reagent. These results show that the alpha-Amylase in whole saliva appears to exist in a macromolecular form which is not dissociated in the presence of sodium dodecyl sulfate (SDS). 1992 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3750/1/Electrophoretic_detection_of_salivary_alpha-amylase_activity.pdf Rahim, Z.H. and Yaacob, H.B. (1992) Electrophoretic detection of salivary alpha-amylase activity. The Journal of Nihon University School of Dentistry, 34 (4). pp. 273-277. ISSN 0029-0432
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic RK Dentistry
spellingShingle RK Dentistry
Rahim, Z.H.
Yaacob, H.B.
Electrophoretic detection of salivary alpha-amylase activity
description Fresh samples of human whole saliva containing approximately 20-40 micrograms protein were analyzed using SDS-polyacrylamide slab gel electrophoresis systems. More than 20 protein bands were revealed by Coomassie Brilliant Blue R 250 staining. Some of the protein bands were shown to be glycoprotein-positive with PAS (periodic acid-Schiff) reagent. The protein bands with alpha-Amylase activity appeared within a molecular weight range of 120,000-180,000, which is 2 to 2.8 times higher than the normal molecular weight reported for alpha-Amylase from parotid saliva, and showed positive staining with PAS reagent. These results show that the alpha-Amylase in whole saliva appears to exist in a macromolecular form which is not dissociated in the presence of sodium dodecyl sulfate (SDS).
format Article
author Rahim, Z.H.
Yaacob, H.B.
author_facet Rahim, Z.H.
Yaacob, H.B.
author_sort Rahim, Z.H.
title Electrophoretic detection of salivary alpha-amylase activity
title_short Electrophoretic detection of salivary alpha-amylase activity
title_full Electrophoretic detection of salivary alpha-amylase activity
title_fullStr Electrophoretic detection of salivary alpha-amylase activity
title_full_unstemmed Electrophoretic detection of salivary alpha-amylase activity
title_sort electrophoretic detection of salivary alpha-amylase activity
publishDate 1992
url http://eprints.um.edu.my/3750/1/Electrophoretic_detection_of_salivary_alpha-amylase_activity.pdf
http://eprints.um.edu.my/3750/
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