Phage N15-based vectors for gene cloning and expression in bacteria and mammalian cells

• Main Authors: Wong, Yin Cheng, Ng, Allan Wee Ren, Chen, Qingwen, Liew, Pei Sheng, Lee, Choon Weng, Sim, Edmund Ui Hang, Narayanan, Kumaran
• Format: Article
• Published: American Chemical Society 2023
• Subjects: TP Chemical technology
• Online Access: http://eprints.um.edu.my/38278/; https://doi.org/10.1021/acssynbio.2c00580909
• Institution: Universiti Malaya

Bacteriophage N15 is the first virus known to deliver linear prophage into Escherichia coli. During its lysogenic cycle, N15 protelomerase (TelN) resolves its telomerase occupancy site (tos) into hairpin telomeres. This protects the N15 prophage from bacterial exonuclease degradation, enabling it to...