The impact of geographical variation in Plasmodium knowlesi Apical Membrane Protein 1 (PkAMA-1) on invasion dynamics of P. knowlesi
Plasmodium knowlesi has emerged as an important zoonotic parasite that causes persistent symptomatic malaria in humans. The signs and symptoms of malaria are attributed to the blood stages of the parasites, which start from the invasion of erythrocytes by the blood stage merozoites. The apical membr...
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my.um.eprints.390662023-11-30T02:48:32Z http://eprints.um.edu.my/39066/ The impact of geographical variation in Plasmodium knowlesi Apical Membrane Protein 1 (PkAMA-1) on invasion dynamics of P. knowlesi Ng, Yee Ling Lee, Wenn Chyau Lau, Yee Ling Fong, Mun Yik R Medicine (General) Plasmodium knowlesi has emerged as an important zoonotic parasite that causes persistent symptomatic malaria in humans. The signs and symptoms of malaria are attributed to the blood stages of the parasites, which start from the invasion of erythrocytes by the blood stage merozoites. The apical membrane protein 1 (AMA-1) plays an important role in the invasion. In this study, we constructed and expressed recombinant PkAMA-1 domain II (PkAMA-1-DII) representing the predominant haplotypes from Peninsular Malaysia and Malaysian Borneo and raised specific antibodies against the recombinant proteins in rabbits. Despite the minor amino acid sequence variation, antibodies raised against haplotypes from Peninsular Malaysia and Malaysian Borneo demonstrated different invasion inhibition (46.81% and 39.45%, respectively) to P. knowlesi A1-H.1, a reference strain derived from Peninsular Malaysia. Here, we demonstrated how a minor variation in a conserved parasite protein could cast a significant impact on parasite invasion biology, suggesting a complex host-switching of P. knowlesi from different locations. This may challenge the implementation of a standardized One Health approach against the transmission of knowlesi malaria. MDPI 2023-01 Article PeerReviewed Ng, Yee Ling and Lee, Wenn Chyau and Lau, Yee Ling and Fong, Mun Yik (2023) The impact of geographical variation in Plasmodium knowlesi Apical Membrane Protein 1 (PkAMA-1) on invasion dynamics of P. knowlesi. Tropical Medicine and Infectious Disease, 8 (1). ISSN 2414-6366, DOI https://doi.org/10.3390/tropicalmed8010056 <https://doi.org/10.3390/tropicalmed8010056>. 10.3390/tropicalmed8010056 |
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R Medicine (General) Ng, Yee Ling Lee, Wenn Chyau Lau, Yee Ling Fong, Mun Yik The impact of geographical variation in Plasmodium knowlesi Apical Membrane Protein 1 (PkAMA-1) on invasion dynamics of P. knowlesi |
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Plasmodium knowlesi has emerged as an important zoonotic parasite that causes persistent symptomatic malaria in humans. The signs and symptoms of malaria are attributed to the blood stages of the parasites, which start from the invasion of erythrocytes by the blood stage merozoites. The apical membrane protein 1 (AMA-1) plays an important role in the invasion. In this study, we constructed and expressed recombinant PkAMA-1 domain II (PkAMA-1-DII) representing the predominant haplotypes from Peninsular Malaysia and Malaysian Borneo and raised specific antibodies against the recombinant proteins in rabbits. Despite the minor amino acid sequence variation, antibodies raised against haplotypes from Peninsular Malaysia and Malaysian Borneo demonstrated different invasion inhibition (46.81% and 39.45%, respectively) to P. knowlesi A1-H.1, a reference strain derived from Peninsular Malaysia. Here, we demonstrated how a minor variation in a conserved parasite protein could cast a significant impact on parasite invasion biology, suggesting a complex host-switching of P. knowlesi from different locations. This may challenge the implementation of a standardized One Health approach against the transmission of knowlesi malaria. |
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Article |
author |
Ng, Yee Ling Lee, Wenn Chyau Lau, Yee Ling Fong, Mun Yik |
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Ng, Yee Ling Lee, Wenn Chyau Lau, Yee Ling Fong, Mun Yik |
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Ng, Yee Ling |
title |
The impact of geographical variation in Plasmodium knowlesi Apical Membrane Protein 1 (PkAMA-1) on invasion dynamics of P. knowlesi |
title_short |
The impact of geographical variation in Plasmodium knowlesi Apical Membrane Protein 1 (PkAMA-1) on invasion dynamics of P. knowlesi |
title_full |
The impact of geographical variation in Plasmodium knowlesi Apical Membrane Protein 1 (PkAMA-1) on invasion dynamics of P. knowlesi |
title_fullStr |
The impact of geographical variation in Plasmodium knowlesi Apical Membrane Protein 1 (PkAMA-1) on invasion dynamics of P. knowlesi |
title_full_unstemmed |
The impact of geographical variation in Plasmodium knowlesi Apical Membrane Protein 1 (PkAMA-1) on invasion dynamics of P. knowlesi |
title_sort |
impact of geographical variation in plasmodium knowlesi apical membrane protein 1 (pkama-1) on invasion dynamics of p. knowlesi |
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MDPI |
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2023 |
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http://eprints.um.edu.my/39066/ |
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1784511860643463168 |