Enhanced large-scale production of recombinant phytase in E. coli DH5 a: Medium components optimization and thermodynamic studies

• Main Authors: Nuge, Tamrin, Hayyan, Adeeb, Elgharbawy, Amal A. M., Salleh, Hamzah Mohd., Yong, Yap Jun, Kamarudin, Ainul F., Hizaddin, Hanee F., Hashim, Yumi Zuhanis Has-Yun, Liu, Xiaoling, Saleh, Jehad, Daoud, Jamal Ibrahim, Aljohani, Abdullah S. M., Alhumaydhi, Fahad A., Zulkifli, M. Y., Nor, Mohd Roslan Mohd, Al Abdulmonem, Waleed
• Format: Article
• Published: Elsevier 2023
• Subjects: T Technology (General); TP Chemical technology
• Online Access: http://eprints.um.edu.my/39211/
• Institution: Universiti Malaya

Medium components and their solubility for improvement of recombinant phytase production in Escherichia coli DH5a. was optimized and reported in this study. Solubility study was supported by the com-putational works conducted using COSMO-RS, a quick tool to predict the thermophysical and chemical pr...