Biosynthesised silver nanoparticles loading onto biphasic calcium phosphate for antibacterial and bone tissue engineering applications

Biphasic calcium phosphate (BCP) serves as one of the substitutes for bone as it consists of an intimate mixture of beta-tricalcium phosphate (beta-TCP) and hydroxyapatite (HAP) in different ratios. BCP, because of its inbuilt properties such as osteoconductivity, biocompatibility, and biostability...

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Main Authors: Padmanabhan, Varun Prasath, Sivashanmugam, Pugalmani, Kulandaivelu, Ravichandran, Sagadevan, Suresh, Sridevi, Balu, Govindasamy, Rajakumar, Thiruvengadam, Muthu
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Published: MDPI 2022
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Online Access:http://eprints.um.edu.my/40301/
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spelling my.um.eprints.403012023-11-23T04:29:59Z http://eprints.um.edu.my/40301/ Biosynthesised silver nanoparticles loading onto biphasic calcium phosphate for antibacterial and bone tissue engineering applications Padmanabhan, Varun Prasath Sivashanmugam, Pugalmani Kulandaivelu, Ravichandran Sagadevan, Suresh Sridevi, Balu Govindasamy, Rajakumar Thiruvengadam, Muthu RM Therapeutics. Pharmacology Biphasic calcium phosphate (BCP) serves as one of the substitutes for bone as it consists of an intimate mixture of beta-tricalcium phosphate (beta-TCP) and hydroxyapatite (HAP) in different ratios. BCP, because of its inbuilt properties such as osteoconductivity, biocompatibility, and biostability in several clinical models serves as a bone substituent for orthopedic applications. Therefore, the present study aimed to assess the effectiveness of silver (Ag) nanoparticles (NPs) combined with BCP composites for the orthopedic sector of bone tissue regeneration and growth. In this regard, we first synthesized Ag-BCP microclusters by the double-emulsion method and then characterized the composite for various physicochemical properties, including the crystallinity and crystal structure, bonding and functionality, porosity, morphology, surface charges, topography, and thermal stability. In addition, the antibacterial activity of Ag-BCP was tested against gram-positive and gram-negative microorganisms such as Staphylococcus aureus, Candida albicans, and Escherichia coli. Finally, the cytocompatibility of Ag-BCP was confirmed against the fibroblast cells in vitro. MDPI 2022-12 Article PeerReviewed Padmanabhan, Varun Prasath and Sivashanmugam, Pugalmani and Kulandaivelu, Ravichandran and Sagadevan, Suresh and Sridevi, Balu and Govindasamy, Rajakumar and Thiruvengadam, Muthu (2022) Biosynthesised silver nanoparticles loading onto biphasic calcium phosphate for antibacterial and bone tissue engineering applications. Antibiotics-Basel, 11 (12). ISSN 2079-6382, DOI https://doi.org/10.3390/antibiotics11121780 <https://doi.org/10.3390/antibiotics11121780>. 10.3390/antibiotics11121780
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic RM Therapeutics. Pharmacology
spellingShingle RM Therapeutics. Pharmacology
Padmanabhan, Varun Prasath
Sivashanmugam, Pugalmani
Kulandaivelu, Ravichandran
Sagadevan, Suresh
Sridevi, Balu
Govindasamy, Rajakumar
Thiruvengadam, Muthu
Biosynthesised silver nanoparticles loading onto biphasic calcium phosphate for antibacterial and bone tissue engineering applications
description Biphasic calcium phosphate (BCP) serves as one of the substitutes for bone as it consists of an intimate mixture of beta-tricalcium phosphate (beta-TCP) and hydroxyapatite (HAP) in different ratios. BCP, because of its inbuilt properties such as osteoconductivity, biocompatibility, and biostability in several clinical models serves as a bone substituent for orthopedic applications. Therefore, the present study aimed to assess the effectiveness of silver (Ag) nanoparticles (NPs) combined with BCP composites for the orthopedic sector of bone tissue regeneration and growth. In this regard, we first synthesized Ag-BCP microclusters by the double-emulsion method and then characterized the composite for various physicochemical properties, including the crystallinity and crystal structure, bonding and functionality, porosity, morphology, surface charges, topography, and thermal stability. In addition, the antibacterial activity of Ag-BCP was tested against gram-positive and gram-negative microorganisms such as Staphylococcus aureus, Candida albicans, and Escherichia coli. Finally, the cytocompatibility of Ag-BCP was confirmed against the fibroblast cells in vitro.
format Article
author Padmanabhan, Varun Prasath
Sivashanmugam, Pugalmani
Kulandaivelu, Ravichandran
Sagadevan, Suresh
Sridevi, Balu
Govindasamy, Rajakumar
Thiruvengadam, Muthu
author_facet Padmanabhan, Varun Prasath
Sivashanmugam, Pugalmani
Kulandaivelu, Ravichandran
Sagadevan, Suresh
Sridevi, Balu
Govindasamy, Rajakumar
Thiruvengadam, Muthu
author_sort Padmanabhan, Varun Prasath
title Biosynthesised silver nanoparticles loading onto biphasic calcium phosphate for antibacterial and bone tissue engineering applications
title_short Biosynthesised silver nanoparticles loading onto biphasic calcium phosphate for antibacterial and bone tissue engineering applications
title_full Biosynthesised silver nanoparticles loading onto biphasic calcium phosphate for antibacterial and bone tissue engineering applications
title_fullStr Biosynthesised silver nanoparticles loading onto biphasic calcium phosphate for antibacterial and bone tissue engineering applications
title_full_unstemmed Biosynthesised silver nanoparticles loading onto biphasic calcium phosphate for antibacterial and bone tissue engineering applications
title_sort biosynthesised silver nanoparticles loading onto biphasic calcium phosphate for antibacterial and bone tissue engineering applications
publisher MDPI
publishDate 2022
url http://eprints.um.edu.my/40301/
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