Molecular identification of human hookworm infections in economically disadvantaged communities in peninsular Malaysia

Species identification of human hookworm infections among eight communities in rural areas of Peninsular Malaysia was determined during 2009-2011. Fecal samples were examined by microscopy and subsequently, the internal transcribed spacer 2 and 28S ribosomal RNA region of Necator americanus and Ancy...

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Main Authors: Ngui, R., Ching, L.S., Kai, T.T., Roslan, M.A., Lim, Y.A.L.
Format: Article
Published: 2012
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Online Access:http://eprints.um.edu.my/4049/
http://www.ncbi.nlm.nih.gov/pubmed/22556084
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spelling my.um.eprints.40492012-12-05T03:53:26Z http://eprints.um.edu.my/4049/ Molecular identification of human hookworm infections in economically disadvantaged communities in peninsular Malaysia Ngui, R. Ching, L.S. Kai, T.T. Roslan, M.A. Lim, Y.A.L. R Medicine Species identification of human hookworm infections among eight communities in rural areas of Peninsular Malaysia was determined during 2009-2011. Fecal samples were examined by microscopy and subsequently, the internal transcribed spacer 2 and 28S ribosomal RNA region of Necator americanus and Ancylostoma spp. were sequenced. Overall, 9.1 (58 of 634) were identified positive by microscopy for hookworm infection, and 47 (81.0) of 58 were successfully amplified and sequenced. Sequence comparison found that N. americanus (87.2) was the most predominant hookworm identified, followed by Ancylostoma ceylanicum (23.4). No A. duodenale infection was detected in this study. Detection of A. ceylanicum in humans highlighted the zoonotic transmission among humans living near dogs. Thus, implementation of effective control measures for hookworm infections in future should seriously consider this zoonotic implication. 2012 Article PeerReviewed Ngui, R. and Ching, L.S. and Kai, T.T. and Roslan, M.A. and Lim, Y.A.L. (2012) Molecular identification of human hookworm infections in economically disadvantaged communities in peninsular Malaysia. American Journal of Tropical Medicine and Hygiene, 86 (5). pp. 837-842. ISSN 0002-9637 http://www.ncbi.nlm.nih.gov/pubmed/22556084 22556084
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Ngui, R.
Ching, L.S.
Kai, T.T.
Roslan, M.A.
Lim, Y.A.L.
Molecular identification of human hookworm infections in economically disadvantaged communities in peninsular Malaysia
description Species identification of human hookworm infections among eight communities in rural areas of Peninsular Malaysia was determined during 2009-2011. Fecal samples were examined by microscopy and subsequently, the internal transcribed spacer 2 and 28S ribosomal RNA region of Necator americanus and Ancylostoma spp. were sequenced. Overall, 9.1 (58 of 634) were identified positive by microscopy for hookworm infection, and 47 (81.0) of 58 were successfully amplified and sequenced. Sequence comparison found that N. americanus (87.2) was the most predominant hookworm identified, followed by Ancylostoma ceylanicum (23.4). No A. duodenale infection was detected in this study. Detection of A. ceylanicum in humans highlighted the zoonotic transmission among humans living near dogs. Thus, implementation of effective control measures for hookworm infections in future should seriously consider this zoonotic implication.
format Article
author Ngui, R.
Ching, L.S.
Kai, T.T.
Roslan, M.A.
Lim, Y.A.L.
author_facet Ngui, R.
Ching, L.S.
Kai, T.T.
Roslan, M.A.
Lim, Y.A.L.
author_sort Ngui, R.
title Molecular identification of human hookworm infections in economically disadvantaged communities in peninsular Malaysia
title_short Molecular identification of human hookworm infections in economically disadvantaged communities in peninsular Malaysia
title_full Molecular identification of human hookworm infections in economically disadvantaged communities in peninsular Malaysia
title_fullStr Molecular identification of human hookworm infections in economically disadvantaged communities in peninsular Malaysia
title_full_unstemmed Molecular identification of human hookworm infections in economically disadvantaged communities in peninsular Malaysia
title_sort molecular identification of human hookworm infections in economically disadvantaged communities in peninsular malaysia
publishDate 2012
url http://eprints.um.edu.my/4049/
http://www.ncbi.nlm.nih.gov/pubmed/22556084
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