Synergistic effects of plant growth regulators and elicitors on alpha-humulene and zerumbone production in Zingiber zerumbet smith adventitious root cultures
Zingiber zerumbet, also known as `Lempoyang', possesses various phytomedicinal properties, such as anticancer, antimicrobial, anti-inflammatory, antiulcer, and antioxidant properties. Secondary metabolites possessing such properties i.e., zerumbone and alpha-humulene, are found dominantly in th...
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| Main Authors: | , , |
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| Format: | Article |
| Published: |
MDPI
2022
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/41550/ |
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| Institution: | Universiti Malaya |
| Summary: | Zingiber zerumbet, also known as `Lempoyang', possesses various phytomedicinal properties, such as anticancer, antimicrobial, anti-inflammatory, antiulcer, and antioxidant properties. Secondary metabolites possessing such properties i.e., zerumbone and alpha-humulene, are found dominantly in the plant rhizome. Synergistic effects of plant growth hormones and elicitors on in vitro alpha-humulene and zerumbone production, and biomass growth, in adventitious root culture (AdRC) of Z. zerumbet cultivated in a two-stage culture are reported. The culture was induced by supplementation of 1.0 mg/L NAA and 2.0 mg/L IBA (dark), and subsequently maintained in medium supplemented with 1 mg/L NAA and 3 mg/L BAP (16:08 light-dark cycle), yielded the production of zerumbone at 3440 +/- 168 mu g/g and alpha-humulene at 3759 +/- 798 mu g/g. Synergistic elicitation by 400 mu M methyl jasmonate (MeJa) and 400 mu M salicylic acid (SA) resulted in a 13-fold increase in zerumbone (43,000 +/- 200 mu g/g), while 400 mu M MeJa and 600 mu M SA produced a 4.3-fold increase in alpha-humulene (15,800 +/- 5100 mu g/g) compared to control. |
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