Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia

Staphylococcus aureus is a persistent human pathogen responsible for a variety of infections ranging from soft-tissue infections to bacteremia. The objective of this study was to determine genetic relatedness between methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA)...

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Main Authors: Lim, K.T., Yeo, C.C., Suhaili, Z., Thong, Kwai Lin
Format: Article
Language:English
Published: Institute of Infectious Diseases 2012
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Online Access:http://eprints.um.edu.my/5651/1/Comparison_of_Methicillin-Resistant_and_Methicillin-Sensitive_Staphylococcus_aureus_Strains_Isolated_from_a_Tertiary_Hospital_in_Terengganu%2C_Malaysia.pdf
http://eprints.um.edu.my/5651/
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spelling my.um.eprints.56512018-10-15T05:12:02Z http://eprints.um.edu.my/5651/ Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia Lim, K.T. Yeo, C.C. Suhaili, Z. Thong, Kwai Lin Q Science (General) QR Microbiology Staphylococcus aureus is a persistent human pathogen responsible for a variety of infections ranging from soft-tissue infections to bacteremia. The objective of this study was to determine genetic relatedness between methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) strains. We isolated 35 MRSA and 21 MSSA strains from sporadic cases at the main tertiary hospital in Terengganu, Malaysia, screening them for the presence of virulence genes. Their genetic relatedness was determined by accessory gene regulator (agr) types, PCR-restriction fragment length polymorphism (RFLP) of the coa gene, pulsed-field gel electrophoresis (PFGE), S. aureus protein A (spa), and multilocus-sequence typing (MLST). We found that 57z of MRSA and 43z of MSSA strains harbored enterotoxin genes. The majority (87.5z) of the strains were agr type I. PCR-RFLP and PFGE genotyping of the coa gene revealed that MRSA strains were genetically related, whereas MSSA strains had higher heterogeneity. The combined genotype, MLST-spa type ST239-t037, was shared amongMRSA and MSSA strains, indicating thatMRSA strains could have evolved from MSSA strains. Two combined MLST-spa types were present in MRSA strains, whereas 7 different MLST-spa types were detected in MSSA strains, including 2 combined types (ST779-t878 and ST1179-t267) that have not been reported in Malaysia. In conclusion, enterotoxin genes were more prevalent in MRSA than in MSSA strains in the Terengganu hospital. The MSSA strains were genetically more diverse than the MRSA strains. Institute of Infectious Diseases 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5651/1/Comparison_of_Methicillin-Resistant_and_Methicillin-Sensitive_Staphylococcus_aureus_Strains_Isolated_from_a_Tertiary_Hospital_in_Terengganu%2C_Malaysia.pdf Lim, K.T. and Yeo, C.C. and Suhaili, Z. and Thong, Kwai Lin (2012) Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia. Japanese Journal of Infectious Diseases, 65 (6). p. 502. ISSN 1344-6304
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Lim, K.T.
Yeo, C.C.
Suhaili, Z.
Thong, Kwai Lin
Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia
description Staphylococcus aureus is a persistent human pathogen responsible for a variety of infections ranging from soft-tissue infections to bacteremia. The objective of this study was to determine genetic relatedness between methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) strains. We isolated 35 MRSA and 21 MSSA strains from sporadic cases at the main tertiary hospital in Terengganu, Malaysia, screening them for the presence of virulence genes. Their genetic relatedness was determined by accessory gene regulator (agr) types, PCR-restriction fragment length polymorphism (RFLP) of the coa gene, pulsed-field gel electrophoresis (PFGE), S. aureus protein A (spa), and multilocus-sequence typing (MLST). We found that 57z of MRSA and 43z of MSSA strains harbored enterotoxin genes. The majority (87.5z) of the strains were agr type I. PCR-RFLP and PFGE genotyping of the coa gene revealed that MRSA strains were genetically related, whereas MSSA strains had higher heterogeneity. The combined genotype, MLST-spa type ST239-t037, was shared amongMRSA and MSSA strains, indicating thatMRSA strains could have evolved from MSSA strains. Two combined MLST-spa types were present in MRSA strains, whereas 7 different MLST-spa types were detected in MSSA strains, including 2 combined types (ST779-t878 and ST1179-t267) that have not been reported in Malaysia. In conclusion, enterotoxin genes were more prevalent in MRSA than in MSSA strains in the Terengganu hospital. The MSSA strains were genetically more diverse than the MRSA strains.
format Article
author Lim, K.T.
Yeo, C.C.
Suhaili, Z.
Thong, Kwai Lin
author_facet Lim, K.T.
Yeo, C.C.
Suhaili, Z.
Thong, Kwai Lin
author_sort Lim, K.T.
title Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia
title_short Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia
title_full Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia
title_fullStr Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia
title_full_unstemmed Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia
title_sort comparison of methicillin-resistant and methicillin-sensitive staphylococcus aureus strains isolated from a tertiary hospital in terengganu, malaysia
publisher Institute of Infectious Diseases
publishDate 2012
url http://eprints.um.edu.my/5651/1/Comparison_of_Methicillin-Resistant_and_Methicillin-Sensitive_Staphylococcus_aureus_Strains_Isolated_from_a_Tertiary_Hospital_in_Terengganu%2C_Malaysia.pdf
http://eprints.um.edu.my/5651/
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