Effect of carbenicillin on somatic embryos formation of papaya (Carica papaya L. var. Eksotika I)

• Main Authors: Fhaizal, M., Vilasini, P., Abd Rahman, Noorsaadah, Khalid, Norzulaani
• Format: Article
• Language: English
• Published: Faculty of Science, University of Malaya 2006
• Subjects: QH301 Biology
• Online Access: http://eprints.um.edu.my/5936/1/Effecy_of_carbenicillin_on_somatic_embryos_formation_of_papaya_%28Carica_papaya_L_var_Eksotika_I%29.pdf; http://eprints.um.edu.my/5936/
• Institution: Universiti Malaya

Embryogenic callus could be established from immature embryo of Carica papaya L. var. Eksotika I after 3 - 4 months of culture on Callus Induction (CI) medium. Embryogenic callus was successfully induced (100 ) on CI medium supplemented with 250 mg/L Carbenicillin plus 10 mg/L 2,4-dichlorophenoxyacetic acids (2,4-D). Transparent somatic embryos then proliferated from the light brown friable embryogenic callus. Carbenicillin that was frequently used as an antibiotic to prevent fungal contamination in in-vitro cultures was found to stimulate the growth of somatic embryos of Carica papaya L. var. Eksotika I. Somatic embryos were maintained in a medium containing either reduced or without 2,4-D. The cultures could be maintained for a period of 5 - 6 months with no apparent loss of regenerative potential. The somatic embryos germinated on Germination (G) medium supplemented with 0.2 mg/L 6-Benzylaminopurine (BAP) and 2.0 mg/L 1-Napthaleneacetic acids (NAA) produced high regeneration frequency (88.41 ) with initial development of hypocotyls followed by rapid growth of plantlets. The in vitro shoots were readily rooted in G medium supplemented with 0.5 mg/L Indole-3-butyric acid (IBA) with 75 successful rate.