Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization

Fluorescent in situ hybridization (FISH) was carried out using two different oligonucleotide probes specific for Pseudomonas spp. and Acinetobacter spp. These probes were tested against different organisms and were found to be highly specific. Sensitivity testing showed that the probes were able to...

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Main Authors:	Wong, E.H., Subramaniam, G., Navaratnam, P., Sekaran, S.D.
Format:	Article
Published:	Medknow Publications 2007
Subjects:	RC Internal medicine
Online Access:	http://eprints.um.edu.my/723/ http://www.ncbi.nlm.nih.gov/pubmed/18087092
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Institution:	Universiti Malaya

id	my.um.eprints.723
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spelling	my.um.eprints.7232019-07-16T05:49:02Z http://eprints.um.edu.my/723/ Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization Wong, E.H. Subramaniam, G. Navaratnam, P. Sekaran, S.D. RC Internal medicine Fluorescent in situ hybridization (FISH) was carried out using two different oligonucleotide probes specific for Pseudomonas spp. and Acinetobacter spp. These probes were tested against different organisms and were found to be highly specific. Sensitivity testing showed that the probes were able to detect as low as 10 3 CFU/mL. In addition, FISH was carried out directly on positive blood culture samples and the detection of microorganisms took less than 2 h. We believe that FISH is a rapid method that can be used as a routine laboratory diagnostic technique for the detection of Acinetobacter spp. and Pseudomonas spp. in clinical samples. Medknow Publications 2007-10 Article PeerReviewed Wong, E.H. and Subramaniam, G. and Navaratnam, P. and Sekaran, S.D. (2007) Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization. Indian Journal of Medical Microbiology, 25 (4). pp. 391-4. ISSN 0255-0857 http://www.ncbi.nlm.nih.gov/pubmed/18087092 18087092
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	RC Internal medicine
spellingShingle	RC Internal medicine Wong, E.H. Subramaniam, G. Navaratnam, P. Sekaran, S.D. Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization
description	Fluorescent in situ hybridization (FISH) was carried out using two different oligonucleotide probes specific for Pseudomonas spp. and Acinetobacter spp. These probes were tested against different organisms and were found to be highly specific. Sensitivity testing showed that the probes were able to detect as low as 10 3 CFU/mL. In addition, FISH was carried out directly on positive blood culture samples and the detection of microorganisms took less than 2 h. We believe that FISH is a rapid method that can be used as a routine laboratory diagnostic technique for the detection of Acinetobacter spp. and Pseudomonas spp. in clinical samples.
format	Article
author	Wong, E.H. Subramaniam, G. Navaratnam, P. Sekaran, S.D.
author_facet	Wong, E.H. Subramaniam, G. Navaratnam, P. Sekaran, S.D.
author_sort	Wong, E.H.
title	Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization
title_short	Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization
title_full	Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization
title_fullStr	Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization
title_full_unstemmed	Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization
title_sort	rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization
publisher	Medknow Publications
publishDate	2007
url	http://eprints.um.edu.my/723/ http://www.ncbi.nlm.nih.gov/pubmed/18087092
_version_	1643686614169288704

Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization

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