Localisation of citrullinated and carbamylated proteins in the inflamed gingival tissues of rheumatoid arthritis / Lee Yin Hui
Background: Antibodies to citrullinated proteins are widely used as diagnostic marker for rheumatoid arthritis (RA). Antibodies against carbamylated proteins were also suggested to be used as a potential biomarker for RA. It has been speculated that citrullination and carbamylation occur in infla...
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| Format: | Thesis |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://studentsrepo.um.edu.my/11224/4/yin_hui.pdf http://studentsrepo.um.edu.my/11224/ |
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| Institution: | Universiti Malaya |
| Summary: | Background: Antibodies to citrullinated proteins are widely used as diagnostic marker
for rheumatoid arthritis (RA). Antibodies against carbamylated proteins were also
suggested to be used as a potential biomarker for RA. It has been speculated that
citrullination and carbamylation occur in inflamed periodontium which can be a source
of autoantibodies to induce immune responses resulting in the development and
progression of RA. Aim: To determine the presence and location of citrullinated and
carbamylated proteins in the gingival tissues and compare their abundance in
periodontitis (PD) participants with and without RA. Materials and Methods: Gingival
tissue samples of healthy control (n = 5), PD with RA (n = 5) and PD without RA (n = 5)
were collected. Specimens were formalin-fixed, paraffin embedded and sectioned at 4
µm. The tissue sections were analysed for the presence of citrullinated and
carbamylated proteins by immunohistochemistry. Semi-quantitative analysis was
performed to quantify and compare the protein abundance between groups. Results:
Number of cells containing citrullinated and carbamylated proteins with higher intensity
was markedly increased in gingival tissues from PD with or without RA in comparison
with healthy controls. Conclusion: Inflamed gingival tissue is a potential extra-articular
source of citrullinated and carbamylated proteins. The extent to which these proteins
contribute to the pathogenesis of RA warrants further elucidation. |
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