Antibactertial property and toxcity study of medicinal plant Tinospora Crispa l. / Altammar Khadijah Ahmed Y.

Tinospora crispa is a medically important herb used for centuries in traditional medicine for the treatment of various ailments. The aim of this study was to investigate the safety of the aqueous and alcoholic extract of Tinospora crispa by determining its toxicological effects after acute administr...

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Main Author: Altammar Khadijah, Ahmed Y.
Format: Thesis
Published: 2010
Subjects:
Online Access:http://studentsrepo.um.edu.my/6214/1/Introduction_to_Appendices.pdf
http://studentsrepo.um.edu.my/6214/2/Title%2BAbstract%2B_contents.pdf
http://studentsrepo.um.edu.my/6214/
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Institution: Universiti Malaya
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spelling my.um.stud.62142020-02-19T22:02:10Z Antibactertial property and toxcity study of medicinal plant Tinospora Crispa l. / Altammar Khadijah Ahmed Y. Altammar Khadijah, Ahmed Y. SB Plant culture Tinospora crispa is a medically important herb used for centuries in traditional medicine for the treatment of various ailments. The aim of this study was to investigate the safety of the aqueous and alcoholic extract of Tinospora crispa by determining its toxicological effects after acute administration in rats, and to investigate the antibacterial activity of both extracts. Disc diffusion and broth dilution methods were carried out in determining the antibacterial effects of the plant extract on the pure cultures of clinical isolates of 8 MRSA and S. aureus ATCC strain. The disc-diffusion method of the ethanolic extracts showed inhibition zone on the tested organisms. The minimum inhibitory concentration (MIC) of the ethanolic extracts on the tested organisms after 48 hours was 25 mg/ml while that of aqueous extract has no effect on majority of the test organisms. Acute toxicity study with a higher dose of 4 g/kg ethanolic extract and 2 g/kg aqueous extracts did not manifest any toxicological signs in rats. However, aqueous extract showed mortality at 4 g/kg. No significant changes of biochemical parameters (liver and renal functions) were observed between control groups and surviving rats. In conclusion, it was demonstrated that ethanolic extract of T. crispa is a promising anti-MRSA agent, and the value of LD50 was estimated as greater than administrated dose (4 g/kg). 2010 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/6214/1/Introduction_to_Appendices.pdf application/pdf http://studentsrepo.um.edu.my/6214/2/Title%2BAbstract%2B_contents.pdf Altammar Khadijah, Ahmed Y. (2010) Antibactertial property and toxcity study of medicinal plant Tinospora Crispa l. / Altammar Khadijah Ahmed Y. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/6214/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic SB Plant culture
spellingShingle SB Plant culture
Altammar Khadijah, Ahmed Y.
Antibactertial property and toxcity study of medicinal plant Tinospora Crispa l. / Altammar Khadijah Ahmed Y.
description Tinospora crispa is a medically important herb used for centuries in traditional medicine for the treatment of various ailments. The aim of this study was to investigate the safety of the aqueous and alcoholic extract of Tinospora crispa by determining its toxicological effects after acute administration in rats, and to investigate the antibacterial activity of both extracts. Disc diffusion and broth dilution methods were carried out in determining the antibacterial effects of the plant extract on the pure cultures of clinical isolates of 8 MRSA and S. aureus ATCC strain. The disc-diffusion method of the ethanolic extracts showed inhibition zone on the tested organisms. The minimum inhibitory concentration (MIC) of the ethanolic extracts on the tested organisms after 48 hours was 25 mg/ml while that of aqueous extract has no effect on majority of the test organisms. Acute toxicity study with a higher dose of 4 g/kg ethanolic extract and 2 g/kg aqueous extracts did not manifest any toxicological signs in rats. However, aqueous extract showed mortality at 4 g/kg. No significant changes of biochemical parameters (liver and renal functions) were observed between control groups and surviving rats. In conclusion, it was demonstrated that ethanolic extract of T. crispa is a promising anti-MRSA agent, and the value of LD50 was estimated as greater than administrated dose (4 g/kg).
format Thesis
author Altammar Khadijah, Ahmed Y.
author_facet Altammar Khadijah, Ahmed Y.
author_sort Altammar Khadijah, Ahmed Y.
title Antibactertial property and toxcity study of medicinal plant Tinospora Crispa l. / Altammar Khadijah Ahmed Y.
title_short Antibactertial property and toxcity study of medicinal plant Tinospora Crispa l. / Altammar Khadijah Ahmed Y.
title_full Antibactertial property and toxcity study of medicinal plant Tinospora Crispa l. / Altammar Khadijah Ahmed Y.
title_fullStr Antibactertial property and toxcity study of medicinal plant Tinospora Crispa l. / Altammar Khadijah Ahmed Y.
title_full_unstemmed Antibactertial property and toxcity study of medicinal plant Tinospora Crispa l. / Altammar Khadijah Ahmed Y.
title_sort antibactertial property and toxcity study of medicinal plant tinospora crispa l. / altammar khadijah ahmed y.
publishDate 2010
url http://studentsrepo.um.edu.my/6214/1/Introduction_to_Appendices.pdf
http://studentsrepo.um.edu.my/6214/2/Title%2BAbstract%2B_contents.pdf
http://studentsrepo.um.edu.my/6214/
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