Antifungal activity of Talinum triangulare (Jacq.) willd against Fusarium oxysporum race cubense (FOC)

• Main Author: Erna Erlina Suharto
• Format: Undergraduate Final Project Report
• Published: 2016
• Online Access: http://discol.umk.edu.my/id/eprint/6476/
• Institution: Universiti Malaysia Kelantan

T. triangulare (Jacq.) willd was a medicinal herbs plant that often used traditionally in treating diabetes, painful eyes, cardiovascular diseases, and gastrointestinal disorders. It also consumed traditionally as ‘ulam'. ln Malaysia, this herbs known as Ginseng Jawa. In this study, T. triangulare was scientifically tested to identify whether it can be used to controlling Fusarium wilt or Panama diseases which caused by Fusarium oxysporum race cubense. Different parts of this herb; rhizomes and leaves were extracted to determine which part of plants has the best antifungal activity, which could inhibit the growth of the FoC. Ethanolic extract of leaves and rhizomes parts was preliminary being tested using agar well diffusion method. Minimum inhibitory concentration (MIC) was then determined by using microdilution method. MIC was determined by determining the minimum concentration which can inhibit the growth of FoC.Based on the results obtained, the rhizomes part of this plant has the highest antifungal activity with the average diameter of inhibition of 2.05 cm at concentration of 50 mg/ml. For leaves, the highest average diameter of the inhibition zone was with 1.5 cm at concentration of 70 mg/ml. For determination of MIC, microdilution method was done at the concentration of 10 mg/ml (for both extract). The resultant concentrations were 10 mg/ml, 5 mg/ml, 2.5 mg/ml, 1.25 mg/ml dn 0.625 mg/ml. The results obtained shows that the minimum concentration that can inhibit the growth of FoC was at 0.625 mg/ml for both extracts (rhizomes and leaves).