Determination of suitable microspore stage and callus induction from anthers of Kenaf (Hibiscus Cannabinus l.)

Kenaf (Hibiscus cannabinus L.) is one of the important species of Hibiscus cultivated for fiber. Availability of homozygous parent lines is prerequisite to the use of the heterosis effect reproducible in hybrid breeding. The production of haploid plants by anther culture followed by chromosome doubl...

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Main Authors: Ahmed Mahmood Ibrahim, Fatimah Binti Kayat, Zeti Ermiena Surya Mat Hussin, Dwi Susanto, Mohammed Ariffulah
Format: Non-Indexed Article
Published: 2014
Online Access:http://discol.umk.edu.my/id/eprint/8070/
http://www.hindawi.com/journals/tswj/2014/284342/abs/
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Institution: Universiti Malaysia Kelantan
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spelling my.umk.eprints.80702022-05-23T10:25:08Z http://discol.umk.edu.my/id/eprint/8070/ Determination of suitable microspore stage and callus induction from anthers of Kenaf (Hibiscus Cannabinus l.) Ahmed Mahmood Ibrahim Fatimah Binti Kayat Zeti Ermiena Surya Mat Hussin Dwi Susanto Mohammed Ariffulah Kenaf (Hibiscus cannabinus L.) is one of the important species of Hibiscus cultivated for fiber. Availability of homozygous parent lines is prerequisite to the use of the heterosis effect reproducible in hybrid breeding. The production of haploid plants by anther culture followed by chromosome doubling can be achieved in short period compared with inbred lines by conventional method that requires self pollination of parent material. In this research, the effects of the microspore developmental stage, time of flower collection, various pretreatments, different combinations of hormones, and culture condition on anther culture of KB6 variety of Kenaf were studied. Young flower buds with immature anthers at the appropriate stage of microspore development were sterilized and the anthers were carefully dissected from the flower buds and subjected to various pretreatments and different combinations of hormones like NAA, 2,4-D, Kinetin, BAP, and TDZ to induce callus. The best microspore development stage of the flower buds was about 6–8 mm long collected 1-2 weeks after flower initiation. At that stage, the microspores were at the uninucleate stage which was suitable for culture. The best callus induction frequency was 90% in the optimized semisolid MS medium fortified with 3.0 mg/L BAP + 3.0 mg/L NAA. 2014 Non-Indexed Article NonPeerReviewed Ahmed Mahmood Ibrahim and Fatimah Binti Kayat and Zeti Ermiena Surya Mat Hussin and Dwi Susanto and Mohammed Ariffulah (2014) Determination of suitable microspore stage and callus induction from anthers of Kenaf (Hibiscus Cannabinus l.). The Scientific World Journal, 2014. pp. 1-5. http://www.hindawi.com/journals/tswj/2014/284342/abs/
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Kenaf (Hibiscus cannabinus L.) is one of the important species of Hibiscus cultivated for fiber. Availability of homozygous parent lines is prerequisite to the use of the heterosis effect reproducible in hybrid breeding. The production of haploid plants by anther culture followed by chromosome doubling can be achieved in short period compared with inbred lines by conventional method that requires self pollination of parent material. In this research, the effects of the microspore developmental stage, time of flower collection, various pretreatments, different combinations of hormones, and culture condition on anther culture of KB6 variety of Kenaf were studied. Young flower buds with immature anthers at the appropriate stage of microspore development were sterilized and the anthers were carefully dissected from the flower buds and subjected to various pretreatments and different combinations of hormones like NAA, 2,4-D, Kinetin, BAP, and TDZ to induce callus. The best microspore development stage of the flower buds was about 6–8 mm long collected 1-2 weeks after flower initiation. At that stage, the microspores were at the uninucleate stage which was suitable for culture. The best callus induction frequency was 90% in the optimized semisolid MS medium fortified with 3.0 mg/L BAP + 3.0 mg/L NAA.
format Non-Indexed Article
author Ahmed Mahmood Ibrahim
Fatimah Binti Kayat
Zeti Ermiena Surya Mat Hussin
Dwi Susanto
Mohammed Ariffulah
spellingShingle Ahmed Mahmood Ibrahim
Fatimah Binti Kayat
Zeti Ermiena Surya Mat Hussin
Dwi Susanto
Mohammed Ariffulah
Determination of suitable microspore stage and callus induction from anthers of Kenaf (Hibiscus Cannabinus l.)
author_facet Ahmed Mahmood Ibrahim
Fatimah Binti Kayat
Zeti Ermiena Surya Mat Hussin
Dwi Susanto
Mohammed Ariffulah
author_sort Ahmed Mahmood Ibrahim
title Determination of suitable microspore stage and callus induction from anthers of Kenaf (Hibiscus Cannabinus l.)
title_short Determination of suitable microspore stage and callus induction from anthers of Kenaf (Hibiscus Cannabinus l.)
title_full Determination of suitable microspore stage and callus induction from anthers of Kenaf (Hibiscus Cannabinus l.)
title_fullStr Determination of suitable microspore stage and callus induction from anthers of Kenaf (Hibiscus Cannabinus l.)
title_full_unstemmed Determination of suitable microspore stage and callus induction from anthers of Kenaf (Hibiscus Cannabinus l.)
title_sort determination of suitable microspore stage and callus induction from anthers of kenaf (hibiscus cannabinus l.)
publishDate 2014
url http://discol.umk.edu.my/id/eprint/8070/
http://www.hindawi.com/journals/tswj/2014/284342/abs/
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