Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads

Enzymes serving as biocatalysts and play an important roles in many industrial field. However, the limitation of enzyme usage due to its high cost and unstable conditions of soluble enzyme to harsh conditions lead to findings an alternative to enhance the enzyme efficiency by immobilization (insolub...

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Main Authors: Mimi Sakinah, A. M., Siti Sabrina, M. S.
Format: Conference or Workshop Item
Language:English
English
Published: 2016
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Online Access:http://umpir.ump.edu.my/id/eprint/14760/1/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques.pdf
http://umpir.ump.edu.my/id/eprint/14760/7/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques1.pdf
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Institution: Universiti Malaysia Pahang Al-Sultan Abdullah
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spelling my.ump.umpir.147602018-01-17T06:30:02Z http://umpir.ump.edu.my/id/eprint/14760/ Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads Mimi Sakinah, A. M. Siti Sabrina, M. S. TP Chemical technology Enzymes serving as biocatalysts and play an important roles in many industrial field. However, the limitation of enzyme usage due to its high cost and unstable conditions of soluble enzyme to harsh conditions lead to findings an alternative to enhance the enzyme efficiency by immobilization (insoluble enzyme). The present work reported a combination of immobilization technique of xylanase by entrapment and covalent binding on alginate hydrogel beads. Xylanase enzyme was effectively immobilized within the alginate hydrogel beads by entrapment and covalent binding on the surface of alginate beads using glutaraldehyde as a cross-linked agent. The effects of immobilization parameters include of sodium alginate concentration (% w/v), calcium chloride (M), and glutaraldehyde concentration (% w/v) were studied in order to obtain a better immobilization yield. These effects were studied using one-factor-at-one-time (OFAT) to obtain the best conditions for xylanase immobilization. The analysis of xylanase activity was determined using dinitrosalicyclic (DNS) acid reagent method. Maximal enzyme immobilization yield (>80 %) was achieved at 3.0 % w/v sodium alginate concentration, 0.3 M calcium alginate, and 9 % w/v of glutaraldehyde concentration. The study shows the xylanase can be immobilized efficiently by a combination of immobilization techniques by entrapment and covalent binding on alginate hydrogel beads 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/14760/1/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/14760/7/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques1.pdf Mimi Sakinah, A. M. and Siti Sabrina, M. S. (2016) Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads. In: International Conference in Organic Synthesis 2016 (ICOS2016), 21-24 August 2016 , Kuching, Sarawak. pp. 1-10..
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mimi Sakinah, A. M.
Siti Sabrina, M. S.
Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
description Enzymes serving as biocatalysts and play an important roles in many industrial field. However, the limitation of enzyme usage due to its high cost and unstable conditions of soluble enzyme to harsh conditions lead to findings an alternative to enhance the enzyme efficiency by immobilization (insoluble enzyme). The present work reported a combination of immobilization technique of xylanase by entrapment and covalent binding on alginate hydrogel beads. Xylanase enzyme was effectively immobilized within the alginate hydrogel beads by entrapment and covalent binding on the surface of alginate beads using glutaraldehyde as a cross-linked agent. The effects of immobilization parameters include of sodium alginate concentration (% w/v), calcium chloride (M), and glutaraldehyde concentration (% w/v) were studied in order to obtain a better immobilization yield. These effects were studied using one-factor-at-one-time (OFAT) to obtain the best conditions for xylanase immobilization. The analysis of xylanase activity was determined using dinitrosalicyclic (DNS) acid reagent method. Maximal enzyme immobilization yield (>80 %) was achieved at 3.0 % w/v sodium alginate concentration, 0.3 M calcium alginate, and 9 % w/v of glutaraldehyde concentration. The study shows the xylanase can be immobilized efficiently by a combination of immobilization techniques by entrapment and covalent binding on alginate hydrogel beads
format Conference or Workshop Item
author Mimi Sakinah, A. M.
Siti Sabrina, M. S.
author_facet Mimi Sakinah, A. M.
Siti Sabrina, M. S.
author_sort Mimi Sakinah, A. M.
title Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
title_short Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
title_full Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
title_fullStr Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
title_full_unstemmed Combination of Immobilization Techniques of Xylanase by Entrapment and Covalent Binding on Alginate Hydrogel Beads
title_sort combination of immobilization techniques of xylanase by entrapment and covalent binding on alginate hydrogel beads
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/14760/1/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques.pdf
http://umpir.ump.edu.my/id/eprint/14760/7/fkksa-2016-sabrina-Combination%20of%20Immobilization%20Techniques1.pdf
http://umpir.ump.edu.my/id/eprint/14760/
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