Antimicrobial activities of silver nanoparticles bio-synthesized from diatom Amphora sp.

• Main Authors: Tevan, R., Mugilan, Govindaraju, Saravanan, Jayakumar, Govindan, Natanamurugaraj, Mohd Hasbi, Ab. Rahim, Gaanty Pragas, Maniam, Ichwan, Solachuddin J. A.
• Format: Article
• Language: English
• Published: Research Malaysia Publications 2017
• Subjects: QP Physiology
• Online Access: http://umpir.ump.edu.my/id/eprint/20694/1/Antimicrobial%20activities%20of%20silver%20nanoparticles%20bio-synthesized-fist-2017.pdf; http://umpir.ump.edu.my/id/eprint/20694/; https://www.jesrjournal.com/uploads/2/6/8/1/26810285/027-jesr-176-184.pdf
• Institution: Universiti Malaysia Pahang

A biological method was employed to synthesize silver nanoparticles through marine diatom Amphora sp. Antimicrobial efficacy test against different pathogenic bacteria were performed through synthesized silver nanoparticles. The physio-chemical properties of synthesized silver nanoparticles were studied using analytical techniques such as UV-Vis spectrophotometer, Field Emission Scanning Electron Microscopy (FESEM), Energy-Dispersive X-ray Spectroscopy (EDX) and Fourier Transform Infrared Spectroscopy (FTIR) and X-ray Powder Diffraction (XRD). UV-Vis color intensity study and higher magnification of the Field Emission Scanning Electron Microscopy image showed the synthesized silver nanoparticles were rod shaped with a size range from 42 nm to 46 nm. The synthesized nanoparticles exhibited antibacterial activities in varying magnitudes. About 10 mg/ml of silver nanoparticles were able to inhibit the growth of gram-negative bacteria while gram-positive bacteria were resistant towards similar concentrations of silver nanoparticles.