Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology

• Main Authors: Zatul Iffah, Mohd Arshad, Azura, Amid
• Format: Conference or Workshop Item
• Language: English; English
• Published: 2018
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/25502/1/122.%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf; http://umpir.ump.edu.my/id/eprint/25502/2/122.1%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf; http://umpir.ump.edu.my/id/eprint/25502/; http://dx.doi.org/10.14419/ijet.v7i4.30.22326
• Institution: Universiti Malaysia Pahang

Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel.