Formulation of gel hand sanitizer using response surface methodology

• Main Author: Siti Damia Amanda, Hamzah
• Format: Undergraduates Project Papers
• Language: English
• Published: 2019
• Subjects: RA0421 Public health. Hygiene. Preventive Medicine
• Online Access: http://umpir.ump.edu.my/id/eprint/29003/1/Formulation%20of%20gel%20hand%20sanitizer%20using%20response%20surface.pdf; http://umpir.ump.edu.my/id/eprint/29003/
• Institution: Universiti Malaysia Pahang Al-Sultan Abdullah

Hand sanitizer is introduced as a new alternative way in hand hygiene technique that should be noted among people nowadays. Variety types of skin diseases such as psoriasis, rashes, impetigo and cellulitis are easily get transferred via skin contact, household appliances and many more. New invention of sanitizer is preferable due to its advantages that convenient and save time give a good impact for the consumers compared to hand washing and soap. Due to that case, a research regarding on determining a formulation has been done in order to investigate the best optimization based on alcohol based hand sanitizer with herbal traditional extract. D-Optimum Design (DO) technique via Response Surface Methodology in Design Expert Software is used to justifiy the most suitable condition in order to create an appropriate formulation. Some factors that been used are the ratio between the main Active Pharmaceutical Ingredient, (API) Isopropyl alcohol and piper betel extract. Zone inhibition diameter is used as the indicator to detect the effectiveness between combination of Isopropyl alcohol and piper betel extract towards antimicrobial activity, Gram-Positive bacteria and Gram-Negative bacteria. The values interpreted from Response Surface Methodology are acquired to assist in optimization process throughout a set of data. All the tasks in this experiment are based on the set data given. Agar Well Diffusion method is choose to investigate the effectiveness of each formulation by observing the antimicrobial activity. The results obtained from (ANOVA) will be discuss to make inference in order to achieve the objectives of this research upon selected best ratio of Isopropyl alcohol and Piper betel extract. According to analysis interpreted from Response Surface Methodology, the value ratio of Isopropyl alcohol and Piper betel extract is lie between 63%, validated as the best optimization. In overall, this result depicts that determination value of ratio for Isopropyl alcohol will give a great impact within suitable value ratio compared to the excipients such as can prevent from skin irritation and easy to evaporated, lead to low of sensitivity reaction.