Piper betle leaves extract adversely affect motility of pseudomonas aeruginosa

Pseudomonas aeruginosa is an opportunistic pathogen that orchestrate its disease manifestation via a complex communication system known as quorum sensing (QS). Under the regulation of this complex network of QS system, is a myriad of virulence factors including the flagella and pili that contribute...

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Main Authors: Saiedatul Akmal, Mohamed Sedek, Mohd Azmir, Arifin, Mimi Sakinah, A. M.
Format: Article
Language:English
Published: Penerbit Universiti Malaysia Pahang 2021
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Online Access:http://umpir.ump.edu.my/id/eprint/31167/1/Piper%20betle%20leaves%20extract%20adversely%20affect%20motility%20of%20Pseudomonas%20aeruginosa.pdf
http://umpir.ump.edu.my/id/eprint/31167/
https://doi.org/10.15282/http://dx.doi.org/10.15282/ijets.7.1.2020.1003
https://doi.org/10.15282/http://dx.doi.org/10.15282/ijets.7.1.2020.1003
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Institution: Universiti Malaysia Pahang
Language: English
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spelling my.ump.umpir.311672021-04-28T04:27:26Z http://umpir.ump.edu.my/id/eprint/31167/ Piper betle leaves extract adversely affect motility of pseudomonas aeruginosa Saiedatul Akmal, Mohamed Sedek Mohd Azmir, Arifin Mimi Sakinah, A. M. TP Chemical technology Pseudomonas aeruginosa is an opportunistic pathogen that orchestrate its disease manifestation via a complex communication system known as quorum sensing (QS). Under the regulation of this complex network of QS system, is a myriad of virulence factors including the flagella and pili that contribute to its motility. To establish an infection in a host, P. aeruginosa must first attach itself to host cells with the assistance of flagella and pili and it would be a mere impossible for P. aeruginosa to initiate and establish an infection in the host without those features. In this study, we investigated the motility of P. aeruginosa under the influence of Piper betle leaves extract by growing the bacteria on a semi solid medium and compared the growth spread of the pathogen against the untreated sample. There are three types of motilities that were being investigated, which were swimming, swarming and twitching. In all three assays, a fraction of ethyl acetate extract of P. betle leaves were found to reduce the motility of P. aeruginosa with greatest defect in swimming at more than 80% reduction as compared to the untreated sample. Meanwhile, the fraction partially decreased the swarming and twitching of P. aeruginosa by 58% and 50% reduction respectively, in comparison to the untreated sample. The exhibited defect in the motility of P. aeruginosa proves the anti-QS property of the fraction of ethyl acetate extract of P. betle leaves and could be developed as a potential therapeutic agent against the pathogen. Penerbit Universiti Malaysia Pahang 2021-02-10 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/31167/1/Piper%20betle%20leaves%20extract%20adversely%20affect%20motility%20of%20Pseudomonas%20aeruginosa.pdf Saiedatul Akmal, Mohamed Sedek and Mohd Azmir, Arifin and Mimi Sakinah, A. M. (2021) Piper betle leaves extract adversely affect motility of pseudomonas aeruginosa. International Journal of Engineering Technology And Sciences (IJETS), 7 (1). pp. 26-31. ISSN 2289-697X https://doi.org/10.15282/http://dx.doi.org/10.15282/ijets.7.1.2020.1003 https://doi.org/10.15282/http://dx.doi.org/10.15282/ijets.7.1.2020.1003
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Saiedatul Akmal, Mohamed Sedek
Mohd Azmir, Arifin
Mimi Sakinah, A. M.
Piper betle leaves extract adversely affect motility of pseudomonas aeruginosa
description Pseudomonas aeruginosa is an opportunistic pathogen that orchestrate its disease manifestation via a complex communication system known as quorum sensing (QS). Under the regulation of this complex network of QS system, is a myriad of virulence factors including the flagella and pili that contribute to its motility. To establish an infection in a host, P. aeruginosa must first attach itself to host cells with the assistance of flagella and pili and it would be a mere impossible for P. aeruginosa to initiate and establish an infection in the host without those features. In this study, we investigated the motility of P. aeruginosa under the influence of Piper betle leaves extract by growing the bacteria on a semi solid medium and compared the growth spread of the pathogen against the untreated sample. There are three types of motilities that were being investigated, which were swimming, swarming and twitching. In all three assays, a fraction of ethyl acetate extract of P. betle leaves were found to reduce the motility of P. aeruginosa with greatest defect in swimming at more than 80% reduction as compared to the untreated sample. Meanwhile, the fraction partially decreased the swarming and twitching of P. aeruginosa by 58% and 50% reduction respectively, in comparison to the untreated sample. The exhibited defect in the motility of P. aeruginosa proves the anti-QS property of the fraction of ethyl acetate extract of P. betle leaves and could be developed as a potential therapeutic agent against the pathogen.
format Article
author Saiedatul Akmal, Mohamed Sedek
Mohd Azmir, Arifin
Mimi Sakinah, A. M.
author_facet Saiedatul Akmal, Mohamed Sedek
Mohd Azmir, Arifin
Mimi Sakinah, A. M.
author_sort Saiedatul Akmal, Mohamed Sedek
title Piper betle leaves extract adversely affect motility of pseudomonas aeruginosa
title_short Piper betle leaves extract adversely affect motility of pseudomonas aeruginosa
title_full Piper betle leaves extract adversely affect motility of pseudomonas aeruginosa
title_fullStr Piper betle leaves extract adversely affect motility of pseudomonas aeruginosa
title_full_unstemmed Piper betle leaves extract adversely affect motility of pseudomonas aeruginosa
title_sort piper betle leaves extract adversely affect motility of pseudomonas aeruginosa
publisher Penerbit Universiti Malaysia Pahang
publishDate 2021
url http://umpir.ump.edu.my/id/eprint/31167/1/Piper%20betle%20leaves%20extract%20adversely%20affect%20motility%20of%20Pseudomonas%20aeruginosa.pdf
http://umpir.ump.edu.my/id/eprint/31167/
https://doi.org/10.15282/http://dx.doi.org/10.15282/ijets.7.1.2020.1003
https://doi.org/10.15282/http://dx.doi.org/10.15282/ijets.7.1.2020.1003
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