Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis

• Main Authors: Rohaida, Che Man, Rosli, Md Illias, Aizi Nor Mazila, Ramli, Siti Kholijah, Abdul Mudalip
• Format: Article
• Language: English; English
• Published: Wiley 2022
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/33066/1/17.%20Optimization%20of%20the%20cultural%20conditions%20of%20immobilized%20cells%20for%20enzyme%20excretion%20and%20cell%20lysis.pdf; http://umpir.ump.edu.my/id/eprint/33066/2/17.1%20Optimization%20of%20the%20cultural%20conditions%20of%20immobilized%20cells%20for%20enzyme%20excretion%20and%20cell%20lysis.pdf; http://umpir.ump.edu.my/id/eprint/33066/; https://doi.org/10.1002/ceat.202100425; https://doi.org/10.1002/ceat.202100425
• Institution: Universiti Malaysia Pahang

Recombinant enzyme excretion is essential due to stable and active protein production. However, enzyme excretion, especially in the Escherichia coli system, may increase the occurrence of cell lysis. Thus, cell immobilization was used, and optimization of culture conditions was conducted to increase...