The development of kit system for honey quality quick check from different species of stingless bee honey in Malaysia

• Main Author: Ajit, Azilah
• Format: Research Report
• Language: English
• Published: 2017
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/36439/1/The%20development%20of%20kit%20system%20for%20honey%20quality%20quick%20check%20from%20different%20species%20of%20stingless%20bee%20honey%20in%20Malaysia.wm.pdf; http://umpir.ump.edu.my/id/eprint/36439/
• Institution: Universiti Malaysia Pahang

Stingless bee is a large group of bees in family Apidae that have the ability to produce different types of products that valuable to humankind which are honey, bee bread, and propolis. The growing demand for honey in the market has led to the occurrence of the tampering honey with foreign substances and increases the production of artificial honey. There are lots of laboratory tests for honey adulteration however most of tests are tedious, high cost and complicated. Therefore, there should be a continuously development and testing of alternative methods that would allow faster and easier detection of honey adulteration. This research attempt to develop a simple inexpensive paper-based amperometric biosensor based on Prussian Blue (PB)-modified screen-printed carbon electrodes (SPCEs). To do so, cellulose filter paper was used as immobilization matrix for glucose oxidase (GOx), invertase (INV) and hydrogen peroxidase (HRP) as it was successfully embedded within the fibre matrix of paper via physical adsorption. The paper-based biosensor allowed a small amount (8 μL) of sample solution for both glucose sucrose analysis. The glucose biosensor had a linear calibration range between 0.5 mM to 4.5 mM (R2= 0.9925) and a detection limit of 0.15 mM. For sucrose biosensor the calibration ranges from 0.1 mM to 1.1 mM (R2=0.9897) and detection limit of 0.1 mM. Interference study of selected potential interfering compounds on the biosensor response was investigated. In addition, its performance was demonstrated in the analysis of six honey samples. The results obtained using glucose biosensor corroborated well with high performance liquid chromatographic (HPLC) method however for sucrose biosensor, more study should be done to improve the result obtained as it has more than 70% differences with reference. From this research, it can be concluded that, the prototype sensor to determine sugar adulteration in stingless bee honey was successfully developed.