Enhanced excretion of recombinant cyclodextrin glucanotransferase and cell stability of immobilized recombinant Escherichia coli by reducing tryptone concentration

• Main Authors: Che Man, Rohaida, Abdul Manas, Nor Hasmaliana, Md Illias, Rosli, Abdul Mudalip, S. K., Sulaiman, Siti Zubaidah, Masngut, Nasratun
• Format: Article
• Language: English
• Published: Society of Chemical Industry 2023
• Subjects: TD Environmental technology. Sanitary engineering; TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/37464/1/Enhanced%20excretion%20of%20recombinant%20cyclodextrin%20glucanotransferase%20.pdf; http://umpir.ump.edu.my/id/eprint/37464/; https://doi.org/10.1002/jctb.7367; https://doi.org/10.1002/jctb.7367
• Institution: Universiti Malaysia Pahang

BACKGROUND: Recombinant protein excretion has become a mainstream strategy in reducing downstream processing costs. However, recombinant protein excretion is often bottlenecked by cell lysis and plasmid stability. In the present study, recombinant Escherichia coli cell immobilization was performed o...