A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest

This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and...

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Main Authors: Patricia, Vit, Bajaree, Chuttong, Norhasnida, Zawawi, Maria, Diaz, Jane Van, der Meulen, Hajar F., Ahmad, Francisco A., Tomas-Barberan, Gina, Meccia, Khanchai, Danmek, J. Enrique, Moreno, David W., Roubik, Ortrud Monika, Barth, Dirk W., Lachenmeier, Michael S., Engel
Format: Article
Language:English
Published: Universidade Estadual de Feira de Santana 2022
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Online Access:http://umpir.ump.edu.my/id/eprint/38075/1/A%20Novel%20Integrative%20Methodology%20for%20Research%20on%20Pot-honey%20Variations%20During%20Post-harvest.pdf
http://umpir.ump.edu.my/id/eprint/38075/
https://doi.org/10.13102%2Fsociobiology.v69i4.8251
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spelling my.ump.umpir.380752023-07-26T05:12:44Z http://umpir.ump.edu.my/id/eprint/38075/ A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest Patricia, Vit Bajaree, Chuttong Norhasnida, Zawawi Maria, Diaz Jane Van, der Meulen Hajar F., Ahmad Francisco A., Tomas-Barberan Gina, Meccia Khanchai, Danmek J. Enrique, Moreno David W., Roubik Ortrud Monika, Barth Dirk W., Lachenmeier Michael S., Engel HD Industries. Land use. Labor Q Science (General) This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations. Universidade Estadual de Feira de Santana 2022-11 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/38075/1/A%20Novel%20Integrative%20Methodology%20for%20Research%20on%20Pot-honey%20Variations%20During%20Post-harvest.pdf Patricia, Vit and Bajaree, Chuttong and Norhasnida, Zawawi and Maria, Diaz and Jane Van, der Meulen and Hajar F., Ahmad and Francisco A., Tomas-Barberan and Gina, Meccia and Khanchai, Danmek and J. Enrique, Moreno and David W., Roubik and Ortrud Monika, Barth and Dirk W., Lachenmeier and Michael S., Engel (2022) A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest. Sociobiology, 69 (4). e8251. ISSN 0361-6525. (Published) https://doi.org/10.13102%2Fsociobiology.v69i4.8251 10.13102%2Fsociobiology.v69i4.8251
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic HD Industries. Land use. Labor
Q Science (General)
spellingShingle HD Industries. Land use. Labor
Q Science (General)
Patricia, Vit
Bajaree, Chuttong
Norhasnida, Zawawi
Maria, Diaz
Jane Van, der Meulen
Hajar F., Ahmad
Francisco A., Tomas-Barberan
Gina, Meccia
Khanchai, Danmek
J. Enrique, Moreno
David W., Roubik
Ortrud Monika, Barth
Dirk W., Lachenmeier
Michael S., Engel
A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest
description This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations.
format Article
author Patricia, Vit
Bajaree, Chuttong
Norhasnida, Zawawi
Maria, Diaz
Jane Van, der Meulen
Hajar F., Ahmad
Francisco A., Tomas-Barberan
Gina, Meccia
Khanchai, Danmek
J. Enrique, Moreno
David W., Roubik
Ortrud Monika, Barth
Dirk W., Lachenmeier
Michael S., Engel
author_facet Patricia, Vit
Bajaree, Chuttong
Norhasnida, Zawawi
Maria, Diaz
Jane Van, der Meulen
Hajar F., Ahmad
Francisco A., Tomas-Barberan
Gina, Meccia
Khanchai, Danmek
J. Enrique, Moreno
David W., Roubik
Ortrud Monika, Barth
Dirk W., Lachenmeier
Michael S., Engel
author_sort Patricia, Vit
title A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest
title_short A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest
title_full A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest
title_fullStr A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest
title_full_unstemmed A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest
title_sort novel integrative methodology for research on pot-honey variations during post-harvest
publisher Universidade Estadual de Feira de Santana
publishDate 2022
url http://umpir.ump.edu.my/id/eprint/38075/1/A%20Novel%20Integrative%20Methodology%20for%20Research%20on%20Pot-honey%20Variations%20During%20Post-harvest.pdf
http://umpir.ump.edu.my/id/eprint/38075/
https://doi.org/10.13102%2Fsociobiology.v69i4.8251
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