Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16
Poly (3-hydroxybutyrate) (PHB) is a biodegradable polymer that can be synthesized through bacterial fermentation. In this study, Cupriavidus necator H16 was used to synthesize PHB by using jatropha oil as its sole carbon source. Experiments using 12.5 g/L of jatropha oil with the highest PHB accumul...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
International Academy Publishing (IAP)
2013
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/6483/1/Kinetic_Analysis_on_Cell_Growth_and_Biosynthesis_of_Poly_%283-Hydroxybutyrate%29_%28PHB%29_in_Cupriavidus_Necator_H16.pdf http://umpir.ump.edu.my/id/eprint/6483/3/Kinetic_Analysis-maksudur-2013.pdf http://umpir.ump.edu.my/id/eprint/6483/ http://dx.doi.org/10.7763/IJBBB.2013.V3.268 |
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| Institution: | Universiti Malaysia Pahang |
| Language: | English English |
| Summary: | Poly (3-hydroxybutyrate) (PHB) is a biodegradable polymer that can be synthesized through bacterial fermentation. In this study, Cupriavidus necator H16 was used to synthesize PHB by using jatropha oil as its sole carbon source. Experiments using 12.5 g/L of jatropha oil with the highest PHB accumulation of 8.6 g/L at 61.5hr was used to assess its kinetic pattern. Logistic and Leudeking-Piret model
was used to evaluate the cell growth and PHB accumulation
respectively and the theoretical values obtained corresponded well with the experimental data with slight deviation in cell growth and PHB accumulation at the end of stationary phase.
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