Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source

The increasing non-biodegradable plastic waste materials have created critical need in finding a better replacement to the currently available conventional plastic. Researches have shown great interest in the production of polyhydroxyalkanoate (PHA) biopolymer from bacterial fermentation. Although s...

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Main Author: Abeed Fatima, Mohidin Batcha
Format: Thesis
Language:English
Published: 2014
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Online Access:http://umpir.ump.edu.my/id/eprint/9467/1/Biosynthesis%20of%20poly%20%283-hydroxybutyrate%29%20%28PHB%29%20by%20cupriavidus%20necator%20H16%20from%20jatropha%20oil%20as%20carbon%20source.%20Masters%20thesis%2C%20Universiti%20Malaysia%20Pahang.pdf
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spelling my.ump.umpir.94672023-05-09T07:23:56Z http://umpir.ump.edu.my/id/eprint/9467/ Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source Abeed Fatima, Mohidin Batcha QD Chemistry The increasing non-biodegradable plastic waste materials have created critical need in finding a better replacement to the currently available conventional plastic. Researches have shown great interest in the production of polyhydroxyalkanoate (PHA) biopolymer from bacterial fermentation. Although successful attempts have been made in producing these short to medium-chain length biopolymers, there are still problems in terms of yield and cost effectiveness that needs to be resolved. Poly(3-hydroxybutyrate) (PHB), a homopolymer of PHA is one particular example of bioplastic that are naturally produced by bacteria like Cupriavidus necator sp. By using plant oils such as jatropha oil as an alternative, a higher yield of PHB can be obtained and thus reducing the overall production cost of the biopolymers. In this study, Cupriavidus necator H16 was used to synthesize PHB by using jatropha oil as its sole carbon source. Different variables mainly jatropha oil and urea concentrations, and agitation speed were investigated to determine the optimum condition for microbial fermentation in batch culture. Based on the results, the highest cell dry weight and PHB concentration of 20.1 g/L and 15.5 g/L respectively was obtained when 20 g/L of jatropha oil was used along with 1 g/L of urea at 200 rpm of agitation speed. Ethanol was used as external stress factor and the addition of 1.5% (v/v) ethanol at 38 h had a positive effect with a high PHB yield of 0.987 g PHB/g jatropha oil. The kinetic studies for cell growth rate and PHB production were conducted and the data were fitted with Logistic and Leudeking Piret models. The rate constants were evaluated and the theoretical values were in accordance with the experimental data obtained. Optimization through Response Surface Methodology (RSM) at the condition of 0.9 g/L urea, 23.6 g/L jatropha oil and 251 rpm agitation speed resulted in 5% increase in PHB concentration to 17.92 g/L compared to the previously obtained PHB concentration of 17.05 g/L. The present work has succeeded in obtaining a high yield of PHB from an inexpensive raw material. 2014-02 Thesis NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/9467/1/Biosynthesis%20of%20poly%20%283-hydroxybutyrate%29%20%28PHB%29%20by%20cupriavidus%20necator%20H16%20from%20jatropha%20oil%20as%20carbon%20source.%20Masters%20thesis%2C%20Universiti%20Malaysia%20Pahang.pdf Abeed Fatima, Mohidin Batcha (2014) Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source. Masters thesis, Universiti Malaysia Pahang (Contributors, Thesis advisor: Rahman Khan, Md. Maksudur).
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Abeed Fatima, Mohidin Batcha
Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
description The increasing non-biodegradable plastic waste materials have created critical need in finding a better replacement to the currently available conventional plastic. Researches have shown great interest in the production of polyhydroxyalkanoate (PHA) biopolymer from bacterial fermentation. Although successful attempts have been made in producing these short to medium-chain length biopolymers, there are still problems in terms of yield and cost effectiveness that needs to be resolved. Poly(3-hydroxybutyrate) (PHB), a homopolymer of PHA is one particular example of bioplastic that are naturally produced by bacteria like Cupriavidus necator sp. By using plant oils such as jatropha oil as an alternative, a higher yield of PHB can be obtained and thus reducing the overall production cost of the biopolymers. In this study, Cupriavidus necator H16 was used to synthesize PHB by using jatropha oil as its sole carbon source. Different variables mainly jatropha oil and urea concentrations, and agitation speed were investigated to determine the optimum condition for microbial fermentation in batch culture. Based on the results, the highest cell dry weight and PHB concentration of 20.1 g/L and 15.5 g/L respectively was obtained when 20 g/L of jatropha oil was used along with 1 g/L of urea at 200 rpm of agitation speed. Ethanol was used as external stress factor and the addition of 1.5% (v/v) ethanol at 38 h had a positive effect with a high PHB yield of 0.987 g PHB/g jatropha oil. The kinetic studies for cell growth rate and PHB production were conducted and the data were fitted with Logistic and Leudeking Piret models. The rate constants were evaluated and the theoretical values were in accordance with the experimental data obtained. Optimization through Response Surface Methodology (RSM) at the condition of 0.9 g/L urea, 23.6 g/L jatropha oil and 251 rpm agitation speed resulted in 5% increase in PHB concentration to 17.92 g/L compared to the previously obtained PHB concentration of 17.05 g/L. The present work has succeeded in obtaining a high yield of PHB from an inexpensive raw material.
format Thesis
author Abeed Fatima, Mohidin Batcha
author_facet Abeed Fatima, Mohidin Batcha
author_sort Abeed Fatima, Mohidin Batcha
title Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
title_short Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
title_full Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
title_fullStr Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
title_full_unstemmed Biosynthesis of poly (3-hydroxybutyrate) (PHB) by cupriavidus necator H16 from jatropha oil as carbon source
title_sort biosynthesis of poly (3-hydroxybutyrate) (phb) by cupriavidus necator h16 from jatropha oil as carbon source
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/9467/1/Biosynthesis%20of%20poly%20%283-hydroxybutyrate%29%20%28PHB%29%20by%20cupriavidus%20necator%20H16%20from%20jatropha%20oil%20as%20carbon%20source.%20Masters%20thesis%2C%20Universiti%20Malaysia%20Pahang.pdf
http://umpir.ump.edu.my/id/eprint/9467/
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