Effecfs of light Intensity on the growth and pigment production in Haematococcus Pluvialis
The green microalga Haematococcus pluvialis was cultured with different light intensity to determine the effect on cell growth and pigment production. The best growth among the light intensity sources was achieved at 250 µmol m-2 S-1 with cell density of 18.95x106 cells mr1. Cell growth was signifi...
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Main Author: | |
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Format: | Academic Exercise |
Language: | English |
Published: |
2015
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Subjects: | |
Online Access: | https://eprints.ums.edu.my/id/eprint/17453/1/effect_of_light_intensity_on_the_growth_and_pigment.pdf https://eprints.ums.edu.my/id/eprint/17453/ |
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Institution: | Universiti Malaysia Sabah |
Language: | English |
Summary: | The green microalga Haematococcus pluvialis was cultured with different light intensity to determine the effect on cell growth and pigment production. The best growth among the light intensity sources was achieved at 250 µmol m-2 S-1 with cell density of 18.95x106 cells mr1. Cell growth was significantly higher (p<0.05) in 250 IJmol m-2 S-1 compared to 25, 75,150 and 350 mol m-2 S-1. The pigment analysis was performed using dimethyl-sulphoxide (DMSO). The highest Chlorophyll a content was found at light intensity 350 IJmol m-2 S-1 on the 14th day cultivation. Meanwhile, Chlorophyll b content of 26.295 IJg/m was the highest on the 13th day at 250 IJmol m-2 S-1. Moreover, the total carotenoid content accumulated mostly at 250 IJmol m-2 S-1 and at the highest on 14th day. It was concluded that light intensity were greatly influencing the accumulation of Chlorophyll a and b and total carotenoids of the H. pluvialis. Besides, it also affects the cell density. |
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