Effectiveness of DNase and washing steps in removing dead cells’ DNA for PCR detection of viable Escherichia coli

• Main Authors: Ho, C. F., Teoh, Peik Lin, Vun, L. W.
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2012
• Subjects: GE Environmental Sciences
• Online Access: https://eprints.ums.edu.my/id/eprint/19445/1/Effectiveness%20of%20DNase%20and%20washing%20steps%20in%20removing%20dead%20cells.pdf; https://eprints.ums.edu.my/id/eprint/19445/; https://doi.org/10.1080/00207233.2012.727317
• Institution: Universiti Malaysia Sabah

This study investigates the use of DNase and washing steps in removing dead cells’ DNA during sample preparation and their effect on the detection of viable cells using polymerase chain reaction (PCR). The results indicated that the DNA from heat-killed cells could be completely removed by DNase; th...