Long term Mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging
Mesenchymal stems cells (MSCs) are currently the focus of numerous therapeutic approaches in tissue engineering/repair because of their wide multi-lineage potential and their ability to modulate the immune system response following transplantation. Culturing these cells, while maintaining their mult...
Saved in:
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English English |
Published: |
Royal of Chemistry
2014
|
Subjects: | |
Online Access: | https://eprints.ums.edu.my/id/eprint/19879/1/Long%20Term%20Mesenchymal%20Stem%20Cell%20Culture%20on%20a%20Defined%20Synthetic%20Substrate%20with%20Enzyme%20Free%20Passaging.pdf https://eprints.ums.edu.my/id/eprint/19879/7/Long%20term%20Mesenchymal%20stem%20cell%20culture%20on%20a%20defined%20synthetic%20substrate%20with%20enzyme%20free%20passaging.pdf https://eprints.ums.edu.my/id/eprint/19879/ https://doi.org/10.1016/j.biomaterials.2014.04.013 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universiti Malaysia Sabah |
Language: | English English |
id |
my.ums.eprints.19879 |
---|---|
record_format |
eprints |
spelling |
my.ums.eprints.198792021-01-21T04:07:09Z https://eprints.ums.edu.my/id/eprint/19879/ Long term Mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging Cairnan R.E. Duffy Rong Zhang How, Siew Eng Annamaria Lilienkampf Paul A.De Sousa Mark Bradley QH Natural history Mesenchymal stems cells (MSCs) are currently the focus of numerous therapeutic approaches in tissue engineering/repair because of their wide multi-lineage potential and their ability to modulate the immune system response following transplantation. Culturing these cells, while maintaining their multipotency in vitro, currently relies on biological substrates such as gelatin, collagen and fibronectin. In addition, harvesting cells from these substrates requires enzymatic or chemical treatment, a process that will remove a multitude of cellular surface proteins, clearly an undesirable process if cells are to be used therapeutically. Herein, we applied a high-throughput ‘hydrogel microarray’ screening approach to identify thermo-modulatable substrates which can support hES-MP and ADMSC growth, permit gentle reagent free passaging, whilst maintaining multi-lineage potential. In summary, the hydrogel substrate identified, poly(AEtMA-Cl-co-DEAA) cross-linked with MBA, permitted MSCs to be maintained over 10 passages (each time via thermo-modulation), with the cells retaining expression of MSC associated markers and lineage potency. This chemically defined system allowed the passaging and maintenance of cellular phenotype of this clinically important cell type, in the absence of harsh passaging and the need for biological substrates. Royal of Chemistry 2014 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/19879/1/Long%20Term%20Mesenchymal%20Stem%20Cell%20Culture%20on%20a%20Defined%20Synthetic%20Substrate%20with%20Enzyme%20Free%20Passaging.pdf text en https://eprints.ums.edu.my/id/eprint/19879/7/Long%20term%20Mesenchymal%20stem%20cell%20culture%20on%20a%20defined%20synthetic%20substrate%20with%20enzyme%20free%20passaging.pdf Cairnan R.E. Duffy and Rong Zhang and How, Siew Eng and Annamaria Lilienkampf and Paul A.De Sousa and Mark Bradley (2014) Long term Mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging. Biomaterials Science, 35 (23). pp. 5998-6005. ISSN 2047-4849 https://doi.org/10.1016/j.biomaterials.2014.04.013 |
institution |
Universiti Malaysia Sabah |
building |
UMS Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Malaysia Sabah |
content_source |
UMS Institutional Repository |
url_provider |
http://eprints.ums.edu.my/ |
language |
English English |
topic |
QH Natural history |
spellingShingle |
QH Natural history Cairnan R.E. Duffy Rong Zhang How, Siew Eng Annamaria Lilienkampf Paul A.De Sousa Mark Bradley Long term Mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging |
description |
Mesenchymal stems cells (MSCs) are currently the focus of numerous therapeutic approaches in tissue engineering/repair because of their wide multi-lineage potential and their ability to modulate the immune system response following transplantation. Culturing these cells, while maintaining their multipotency in vitro, currently relies on biological substrates such as gelatin, collagen and fibronectin. In addition, harvesting cells from these substrates requires enzymatic or chemical treatment, a process that will remove a multitude of cellular surface proteins, clearly an undesirable process if cells are to be used therapeutically. Herein, we applied a high-throughput ‘hydrogel microarray’ screening approach to identify thermo-modulatable substrates which can support hES-MP and ADMSC growth, permit gentle reagent free passaging, whilst maintaining multi-lineage potential. In summary, the hydrogel substrate identified, poly(AEtMA-Cl-co-DEAA) cross-linked with MBA, permitted MSCs to be maintained over 10 passages (each time via thermo-modulation), with the cells retaining expression of MSC associated markers and lineage potency. This chemically defined system allowed the passaging and maintenance of cellular phenotype of this clinically important cell type, in the absence of harsh passaging and the need for biological substrates. |
format |
Article |
author |
Cairnan R.E. Duffy Rong Zhang How, Siew Eng Annamaria Lilienkampf Paul A.De Sousa Mark Bradley |
author_facet |
Cairnan R.E. Duffy Rong Zhang How, Siew Eng Annamaria Lilienkampf Paul A.De Sousa Mark Bradley |
author_sort |
Cairnan R.E. Duffy |
title |
Long term Mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging |
title_short |
Long term Mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging |
title_full |
Long term Mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging |
title_fullStr |
Long term Mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging |
title_full_unstemmed |
Long term Mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging |
title_sort |
long term mesenchymal stem cell culture on a defined synthetic substrate with enzyme free passaging |
publisher |
Royal of Chemistry |
publishDate |
2014 |
url |
https://eprints.ums.edu.my/id/eprint/19879/1/Long%20Term%20Mesenchymal%20Stem%20Cell%20Culture%20on%20a%20Defined%20Synthetic%20Substrate%20with%20Enzyme%20Free%20Passaging.pdf https://eprints.ums.edu.my/id/eprint/19879/7/Long%20term%20Mesenchymal%20stem%20cell%20culture%20on%20a%20defined%20synthetic%20substrate%20with%20enzyme%20free%20passaging.pdf https://eprints.ums.edu.my/id/eprint/19879/ https://doi.org/10.1016/j.biomaterials.2014.04.013 |
_version_ |
1760229640427798528 |